College of Biology and Food Engineering, Anhui Polytechnic University, Wuhu, 241000, PR China; Anhui Engineering Laboratory for Industrial Microbiology Molecular Breeding, Wuhu, 241000, PR China.
College of Biology and Food Engineering, Anhui Polytechnic University, Wuhu, 241000, PR China; Anhui Engineering Laboratory for Industrial Microbiology Molecular Breeding, Wuhu, 241000, PR China.
Microb Pathog. 2022 Jul;168:105574. doi: 10.1016/j.micpath.2022.105574. Epub 2022 May 11.
Vibrio parahaemolyticus (V. parahaemolyticus) is a common pathogen in seafood. The use of antibiotics is a primary tool to prevent and control V. parahaemolyticus in the aquaculture industry. However, V. parahaemolyticus combats the damage caused by antibiotics by forming biofilms under certain conditions. In this study, we analyzed the antibacterial effect and the characteristics of V. parahaemolyticus by experimentally determining the minimum inhibitory concentration (MIC) and the fractional inhibitory concentration index (FICI) values of a combination of the Litsea cubeba essential oil (LCEO) and several commonly used V. parahaemolyticus antibiotics. The bactericidal effect of the essential oil alone and essential oil in combination with the antibiotics were evaluated with time-kill curves. The damage to cell membranes and cell walls were assessed by measuring the content of macromolecules and alkaline phosphatase (AKP) released into the supernatant using V. parahaemolyticus ATCC17802 as the experimental strain. The membrane structure was observed by transmission electron microscopy. The results showed that the MIC value of the LCEO was 1,024 μg/mL, and the LCEO FICI values in combination with tetracycline or oxytetracycline hydrochloride was 0.3125 and 0.75, respectively, indicating synergistic and additive effects. Moreover, LCEO inhibited the growth and promoted the removal of biofilms by reducing the content of hydrophobic and extracellular polysaccharides on the cell surface. This study provides a reference for studying the antibacterial activity of LCEO and the combination of antibiotics to prevent and control the formation of biofilms by V. parahaemolyticus.
副溶血弧菌（Vibrio parahaemolyticus）是海产品中的一种常见病原体。在水产养殖行业中，抗生素的使用是预防和控制副溶血弧菌的主要手段。然而，在某些条件下，副溶血弧菌会形成生物膜来对抗抗生素造成的破坏。在本研究中，我们通过实验测定了 Litsea cubeba 精油（LCEO）与几种常用副溶血弧菌抗生素联合使用的最小抑菌浓度（MIC）和部分抑菌浓度指数（FICI）值，分析了其抗菌效果和副溶血弧菌的特性。我们通过时间杀菌曲线评估了精油单独和与抗生素联合使用的杀菌效果。利用副溶血弧菌 ATCC17802 作为实验菌株，通过测量大分子物质和碱性磷酸酶（AKP）释放到上清液中的含量来评估细胞膜和细胞壁的损伤情况。通过透射电子显微镜观察膜结构。结果表明，LCEO 的 MIC 值为 1,024μg/mL，与四环素或盐酸土霉素联合使用时的 LCEO FICI 值分别为 0.3125 和 0.75，表明具有协同和相加作用。此外，LCEO 通过降低细胞表面疏水性和胞外多糖的含量来抑制生物膜的形成并促进其去除。本研究为研究 LCEO 的抗菌活性以及抗生素联合使用预防和控制副溶血弧菌生物膜形成提供了参考。
