GenPhySE, Université de Toulouse, INRAE, ENVT, 31326, Castanet-Tolosan, France.
ASTRE, CIRAD, INRAE, 34398, Montpellier, France.
BMC Res Notes. 2022 May 13;15(1):170. doi: 10.1186/s13104-022-06052-w.
The admixture of domestic pig into wild boar populations is controlled until now, by cytogenetic analysis. Even if a first-generation hybrid animal is discernable because of its 37-chromosome karyotype, the cytogenetic method is not applicable in the case of advanced intercrosses. The aim of this study is therefore to evaluate the use of SNP (Single Nucleotide Polymorphism) markers as an alternative technology to characterize recent or past hybridization between the two sub-species. The final goal would be to develop a molecular diagnostic tool.
The Geneseek Genomic Profiler High-Density porcine beadchip (GGP70KHD, Illumina, USA), comprising 68,516 porcine SNPs, was used on a set of 362 wild boars with diverse chromosomal statuses collected from different areas and breeding environments in France. We generated approximately 62,192-64,046 genotypes per wild boar. The present dataset might be useful for the community (i) for developing molecular tools to evaluate the admixture of domestic pig into wild boar populations, and (ii) for genetic diversity studies including wild boar species or phylogeny analyses of Suidae populations. Raw data files and a processed matrix data file were deposited in the ArrayExpress at European Molecular Biology Laboratory-European Bioinformatics Institute (EMBL-EBI) data portal under accession number E-MTAB-10591.
迄今为止，通过细胞遗传学分析来控制家猪与野猪种群的混合。即使由于其 37 条染色体核型可识别第一代杂交动物，但在杂交程度较高的情况下，细胞遗传学方法并不适用。因此，本研究旨在评估 SNP（单核苷酸多态性）标记作为一种替代技术，用于描述两个亚种之间最近或过去的杂交情况。最终目标是开发一种分子诊断工具。
使用来自法国不同地区和养殖环境的 362 头具有不同染色体状态的野猪，对 Geneseek Genomic Profiler High-Density 猪基因芯片（GGP70KHD，Illumina，美国）进行了测试，该基因芯片包含 68516 个猪 SNP。我们为每头野猪生成了大约 62192-64046 个基因型。本数据集可能对以下领域具有一定的参考价值：（i）开发用于评估家猪与野猪种群混合的分子工具；（ii）用于遗传多样性研究，包括野猪物种或猪科种群的系统发育分析。原始数据文件和处理后的矩阵数据文件已在欧洲分子生物学实验室-欧洲生物信息学研究所（EMBL-EBI）数据门户的 ArrayExpress 中以 E-MTAB-10591 的编号进行了存储。
