Stoddart R W, Herbertson B M
J Med Microbiol. 1978 Aug;11(3):315-24. doi: 10.1099/00222615-11-3-315.
Fluorescein-labelled lectins of known specificities for different sugars were used in an attempt to identify fungi in paraffin sections of surgical and post-mortem material. Aspergillus fumigatus, Blastomyces dermatitidis, Candida albicans, Cryptococcus neoformans, Paracoccidioides brasiliensis and Rhizopus oryzae have been studied with five fluorescein-labelled lectins and with basis of differences in their reactions with these stains. The results accord well with what is known of the chemistry of the organisms and the method offers promise to practising histopathologists.
利用对不同糖类具有已知特异性的荧光素标记凝集素，试图在手术和尸检材料的石蜡切片中鉴定真菌。已使用五种荧光素标记凝集素对烟曲霉、皮炎芽生菌、白色念珠菌、新型隐球菌、巴西副球孢子菌和米根霉进行了研究，并根据它们与这些染色剂反应的差异进行分析。结果与已知的生物体化学性质非常吻合，该方法为执业组织病理学家带来了希望。
