Institute of Pathology, University of Luebeck and University Hospital Schleswig-Holstein, Campus Luebeck, 23538 Luebeck, Germany.
Institute of Pathology, Research Center Borstel, Leibniz Lung Center, 23845 Borstel, Germany.
Int J Mol Sci. 2022 May 3;23(9):5075. doi: 10.3390/ijms23095075.
Head and neck squamous cell carcinomas (HNSCC) are among the most common cancers worldwide and are associated with a poor prognosis for patients. Among HNSCC, those originating in the hypopharynx have the worst prognosis. The histone demethylase LSD1 has been shown to promote cancer initiation, progression, and relapse through various mechanisms and is upregulated in many cancer tissues. LSD1 physically interacts with SNAIL and is required for SNAIL mediated transcriptional repression. Previous studies of the prognostic value of LSD1 in HNSCC have been limited in their analysis of sub-sites, and a correlation between LSD1 and SNAIL has not been shown in HNSCC patient samples. Here we used a large, representative, and clinically well-characterized cohort of 339 HNSCC patients to investigate the co-expression of LSD1 and SNAIL and their prognostic value in all HNSCC using immunohistochemical staining. Elevated LSD1 expression correlated with advanced tumor stage and poor progression-free survival (PFS) in HNSCC originating in the hypopharynx. Overexpression of the transcription factor SNAIL independently correlated with worse overall survival (OS) and PFS in HNSCC in general and prominently in tumors of the hypopharynx. Furthermore, increased LSD1 expression significantly correlated with elevated SNAIL expression in patient samples. Therefore, the presented data implicates LSD1 and SNAIL as independent prognostic biomarkers.
头颈部鳞状细胞癌（HNSCC）是全球最常见的癌症之一，患者预后较差。在 HNSCC 中，起源于下咽的癌症预后最差。组蛋白去甲基化酶 LSD1 通过多种机制促进癌症的发生、进展和复发，并且在许多癌症组织中上调。LSD1 与 SNAIL 物理相互作用，并且是 SNAIL 介导的转录抑制所必需的。以前关于 LSD1 在 HNSCC 中的预后价值的研究在其对亚部位的分析中受到限制，并且 LSD1 和 SNAIL 之间的相关性尚未在 HNSCC 患者样本中显示。在这里，我们使用了一个由 339 名 HNSCC 患者组成的大型、代表性和临床特征良好的队列，通过免疫组织化学染色研究 LSD1 和 SNAIL 的共表达及其在所有 HNSCC 中的预后价值。在上咽起源的 HNSCC 中，LSD1 表达升高与肿瘤晚期和无进展生存期（PFS）较差相关。转录因子 SNAIL 的过表达与总体生存（OS）和 PFS 较差相关，尤其是在下咽肿瘤中。此外，在患者样本中，LSD1 表达的增加与 SNAIL 表达的升高显著相关。因此，所提供的数据表明 LSD1 和 SNAIL 是独立的预后生物标志物。
