Przała J, Grazul A, Wiesak T, Muszyńska A, Rzasa J
Exp Clin Endocrinol. 1986 Dec;88(2):193-9. doi: 10.1055/s-0029-1210596.
Porcine luteal cells were obtained from the corpora lutea on the 13th day of the estrous cycle. The cells were digested with 0.25% trypsin and suspended in the Medium 199 with an addition of 10% calf serum, at a concentration of 5 X 10(4) cells/ml. The cells were incubated with or without 4 and 40 mi.u./ml of oxytocin, 10 and 100 ng/ml of arginine-vasotocin, 1 microgram LH and 50 U/ml hCG. Levels of progesterone, testosterone and estradiol 17 beta were determined with the radioimmunological method, following 6 h incubation. It was found that progesterone secretion under the influence of oxytocine (4 mi.u./ml) was less than in the control group and in the group with LH. Similarly, arginine-vasotocin at a dose of 10 ng/ml inhibited progesterone secretion (P less than 0.05). Higher doses of these peptides had no suppressive effect on the luteal cells. Oxytocin and arginine-vasotocin had no influence on testosterone secretion by luteal cells. However, these cells produced less (P less than 0.05) estradiol 17 beta under the influence of oxytocine than of hCG. The results point to a direct effect of oxytocin and arginine-vasotocin on steroidogenesis in the corpora lutea of cyclic pigs.
在发情周期的第13天从黄体中获取猪黄体细胞。用0.25%的胰蛋白酶消化细胞，并将其悬浮于添加了10%小牛血清的199培养基中，细胞浓度为5×10⁴个细胞/毫升。将细胞与4和40毫国际单位/毫升的催产素、10和100纳克/毫升的精氨酸加压素、1微克促黄体生成素和50国际单位/毫升人绒毛膜促性腺激素一起孵育，有或没有这些物质。孵育6小时后，用放射免疫法测定孕酮、睾酮和雌二醇17β的水平。发现催产素（4毫国际单位/毫升）作用下的孕酮分泌低于对照组和促黄体生成素组。同样，剂量为10纳克/毫升的精氨酸加压素抑制孕酮分泌（P<0.05）。这些肽的更高剂量对黄体细胞没有抑制作用。催产素和精氨酸加压素对黄体细胞的睾酮分泌没有影响。然而，在催产素作用下，这些细胞产生的雌二醇17β比在人绒毛膜促性腺激素作用下少（P<0.05）。结果表明催产素和精氨酸加压素对发情周期母猪黄体中的类固醇生成有直接影响。
