Liu Shuo, Zhang Peng, Miao Yuming, Li Chenmin, Shi Yu-E, Liu Jinhua, Lv Yun-Kai, Wang Zhenguang
Key Laboratory of Chemical Biology of Hebei Province, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, College of Chemistry & Environmental Science, Hebei University, Baoding 071002, China.
Department of Pharmacy, Shenzhen Luohu People's Hospital, No. 47 Youyi Rd, Luohu, Shenzhen 518001, China.
Nanomaterials (Basel). 2022 Apr 22;12(9):1429. doi: 10.3390/nano12091429.
Selective and sensitive identification of paraoxon residue in agricultural products is greatly significant for food safety but remains a challenging task. Herein, a detection platform was developed by integrating Cu nanoclusters (Cu NCs) with MnO nanosheets, where the fluorescence of Cu NCs was effectively quenched. Upon introducing butyrylcholinesterase and butyrylcholine into the system, their hydrolysate, thiocholine, leads to the decomposition of the platform through a reaction between the MnO nanosheets and thiol groups on thiocholine. The electron-rich groups on thiocholine can further promote the fluorescence intensity of Cu NCs through host-guest interactions. Adding paraoxon results in the failure of fluorescence recovery and further promotion, which could be utilized for the quantitative detection of paraoxon, and a limit of detection as low as 0.22 ng/mL can be achieved. The detection platform shows strong tolerance to common interference species, which endows its applications for the detection of paraoxon in vegetables and fruit. These presented results not only open a new door for the functionalization of metal nanoclusters but also offer an inspiring strategy for analytic techniques in nanomedicine and environmental science.
选择性和灵敏地鉴定农产品中的对氧磷残留对于食品安全具有重大意义，但仍然是一项具有挑战性的任务。在此，通过将铜纳米簇（Cu NCs）与MnO纳米片整合开发了一种检测平台，其中Cu NCs的荧光被有效淬灭。在将丁酰胆碱酯酶和丁酰胆碱引入系统后，它们的水解产物硫代胆碱通过MnO纳米片与硫代胆碱上的硫醇基团之间的反应导致平台分解。硫代胆碱上的富电子基团可通过主客体相互作用进一步促进Cu NCs的荧光强度。加入对氧磷会导致荧光恢复和进一步增强失败，这可用于对氧磷的定量检测，检测限低至0.22 ng/mL。该检测平台对常见干扰物质具有很强的耐受性，这使其可应用于蔬菜和水果中对氧磷的检测。这些结果不仅为金属纳米簇的功能化打开了一扇新的大门，也为纳米医学和环境科学中的分析技术提供了一种启发性策略。
