Savaid Medical School, University of Chinese Academy of Sciences, Beijing 101400, China.
National Research Institute for Family Planning, Beijing 100081, China.
Molecules. 2022 Apr 24;27(9):2741. doi: 10.3390/molecules27092741.
Fatty acid synthase (FASN) is highly expressed in multiple types of human cancers and is recognized as one of the targets for treating cancer metastasis. α-Linolenic acid is an omega-3 essential fatty acid and it possesses various biological activities. The present study was designed to reveal the effects of α-linolenic acid on osteosarcoma and to reveal whether the mechanism of α-linolenic acid in anticancer activity may be related to FASN inhibition. The cytotoxicity of α-linolenic acid was assessed in osteosarcoma MG63, 143B, and U2OS cells. Cell viability was detected by the MTT assay. The protein expression level was detected by western blotting. Flow cytometry, Annexin V/propidium iodide dual staining, and Hoechst 33258 staining were performed to assess the apoptotic effects. Wound healing assay was applied to detect the inhibitory effect of α-linolenic acid on osteosarcoma cells migration. The results showed that α-linolenic acid downregulated FASN expression. α-Linolenic acid inhibited osteosarcoma cell proliferation and migration in a dose-dependent manner. In addition, α-linolenic acid regulated endoplasmic reticulum transmembrane receptors and signal protein expression in osteosarcoma cells. The findings of the present study suggested that α-linolenic acid suppresses osteosarcoma cell proliferation and metastasis by inhibiting FASN expression, which provides a basis as a potential target for osteosarcoma treatment.
脂肪酸合酶（FASN）在多种人类癌症中高度表达，被认为是治疗癌症转移的靶点之一。α-亚麻酸是一种ω-3 必需脂肪酸，具有多种生物学活性。本研究旨在揭示 α-亚麻酸对骨肉瘤的影响，并揭示 α-亚麻酸在抗癌活性中的机制是否与 FASN 抑制有关。采用 MTT 法检测 α-亚麻酸对骨肉瘤 MG63、143B 和 U2OS 细胞的细胞毒性。通过 Western blot 检测蛋白表达水平。采用流式细胞术、Annexin V/碘化丙啶双重染色和 Hoechst 33258 染色检测细胞凋亡。采用划痕愈合实验检测 α-亚麻酸对骨肉瘤细胞迁移的抑制作用。结果表明，α-亚麻酸下调 FASN 表达。α-亚麻酸呈剂量依赖性抑制骨肉瘤细胞的增殖和迁移。此外，α-亚麻酸调节骨肉瘤细胞内质网跨膜受体和信号蛋白的表达。本研究结果表明，α-亚麻酸通过抑制 FASN 表达抑制骨肉瘤细胞的增殖和转移，为骨肉瘤的治疗提供了一个潜在的靶点。
