van Duren D B, Vemer H M, Bastiaans B L, Doesburg W H, Willemsen W N, Rolland R
Fertil Steril. 1987 Mar;47(3):456-9. doi: 10.1016/s0015-0282(16)59055-4.
Routine semen analysis and a zona-free hamster ovum sperm penetration assay (SPA) were carried out for 220 men from a group of infertile couples. The grade of sperm motility was determined after a preincubation period. For both the normal and abnormal semen samples, the number of positive SPAs was significantly higher when the postcapacitation motility was normal. This difference was observed also when the routine semen characteristics of density, motility, and morphologic features were considered separately regarding their relationship with the SPA. Motility after preincubation showed the most significant positive correlation with the SPA result. Therefore, the SPA score should be limited to semen samples showing adequate postincubation motility.
对一组不育夫妇中的220名男性进行了常规精液分析和无透明带仓鼠卵精子穿透试验（SPA）。在预孵育期后确定精子活力等级。对于正常和异常精液样本，当获能后活力正常时，阳性SPA的数量显著更高。在分别考虑密度、活力和形态特征等常规精液特征与SPA的关系时，也观察到了这种差异。预孵育后的活力与SPA结果显示出最显著的正相关。因此，SPA评分应限于孵育后显示足够活力的精液样本。
