Laboratory of Applied Organic Chemistry, Faculty of Sciences and Techniques, University Sidi Mohammed Ben Abdellah, B.P. 2202, Fes 30000, Morocco.
Institute of Forensic Sciences of Gendarmerie Royal, B.P. 6597, Rabat 10000, Morocco.
Molecules. 2022 May 3;27(9):2914. doi: 10.3390/molecules27092914.
To ensure the better production and sustainable management of natural resources, a chemometric investigation was conducted to examine the effect of cooperative and harvesting periods on the crop yields and chemical compositions of Spenn essential oils in the Oriental region of Morocco. The samples were collected from three cooperatives over nine time periods from January 2018 to April 2019. The chemical composition of Spenn essential oils was analyzed by gas chromatography coupled with mass spectrometry. The data from this study were processed by multivariate analyses, including principal component analysis (PCA) and hierarchical cluster analysis (HCA). The disc diffusion technique and a determination of the minimal inhibitory concentration were performed to study the antibacterial properties of the oils. Statistical analysis showed that the cooperative and harvest period have a significant effect on yields. The highest yield of essential oil was recorded in April 2019 at cooperative C1. The PCA and the HCA results were divided into two groups: Group A for the summer season and group B for the winter season. The samples collected during summer were characterized by a high amount of 1,8-cineole component and a high yield of essential oil, whereas the samples collected during winter were qualified by a high amount of α-pinene component and a low yield of essential oil. The antibacterial activity of Spenn essential oils showed that ATCC23857 and ATCC 23857 are the most susceptible strains, stopping growth at 1/500 (/). The least susceptible strain is Escherichia coli ATCC25922, with an MIC value corresponding to 1/250 (/). The findings of this study could have a positive economic impact on the exploitation of rosemary in the Oriental region, especially during the best harvest periods, as they indicate how to obtain the best yields of oils richest in 1,8-cineole and α-pinene chemotypes.
为了确保更好地生产和可持续管理自然资源，进行了一项化学计量学研究，以研究合作和收获期对摩洛哥东方地区 Spenn 精油作物产量和化学成分的影响。样品于 2018 年 1 月至 2019 年 4 月期间从三个合作社的九个时间段收集。通过气相色谱-质谱联用分析 Spenn 精油的化学成分。本研究的数据通过多元分析进行处理，包括主成分分析（PCA）和层次聚类分析（HCA）。采用圆盘扩散技术和最小抑菌浓度测定法研究了精油的抗菌性能。统计分析表明，合作和收获期对产量有显著影响。2019 年 4 月在合作社 C1 记录到精油的最高产量。PCA 和 HCA 结果分为两组：A 组为夏季，B 组为冬季。夏季采集的样品以 1,8-桉树脑成分含量高和精油产量高为特征，而冬季采集的样品则以 α-蒎烯成分含量高和精油产量低为特征。Spenn 精油的抗菌活性表明，ATCC23857 和 ATCC23857 是最敏感的菌株，在 1/500（%）时停止生长。最不敏感的菌株是大肠杆菌 ATCC25922，其 MIC 值对应于 1/250（%）。本研究的结果可能对东方地区迷迭香的开发产生积极的经济影响，特别是在最佳收获期，因为它们表明如何获得富含 1,8-桉树脑和α-蒎烯化学型的精油的最佳产量。
