Department of Pediatric Dentistry, College of Dentistry, Dankook University, 119 Dandae-ro, Cheonan 31116, Korea.
Institute of Tissue Regeneration Engineering (ITREN), Dankook University, 119 Dandae-ro, Cheonan 31116, Korea.
Molecules. 2022 May 4;27(9):2927. doi: 10.3390/molecules27092927.
Therapeutic iodoform (CHI) is commonly used as a root-filling material for primary teeth; however, the side effects of iodoform-containing materials, including early root resorption, have been reported. To overcome this problem, a water-soluble iodide (NaI)-incorporated root-filling material was developed. Calcium hydroxide, silicone oil, and NaI were incorporated in different weight proportions (30:30:X), and the resulting material was denoted DX (D5~D30), indicating the NaI content. As a control, iodoform instead of NaI was incorporated at a ratio of 30:30:30, and the material was denoted I30. The physicochemical (flow, film thickness, radiopacity, viscosity, water absorption, solubility, and ion releases) and biological (cytotoxicity, TRAP, ARS, and analysis of osteoclastic markers) properties were determined. The amount of iodine, sodium, and calcium ion releases and the pH were higher in D30 than I30, and the highest level of unknown extracted molecules was detected in I30. In the cell viability test, all groups except 100% D30 showed no cytotoxicity. In the 50% nontoxic extract, D30 showed decreased osteoclast formation compared with I30. In summary, NaI-incorporated materials showed adequate physicochemical properties and low osteoclast formation compared to their iodoform-counterpart. Thus, NaI-incorporated materials may be used as a substitute for iodoform-counterparts in root-filling materials after further (pre)clinical investigation.
治疗性碘仿（CHI）常用于儿童乳牙的根管填充材料；然而，含碘材料的副作用，包括早期牙根吸收，已被报道。为了解决这个问题，开发了一种水溶性碘化物（NaI）的根管填充材料。将氢氧化钙、硅油和 NaI 以不同的重量比例（30:30:X）混合，得到的材料分别用 DX（D5~D30）表示，其中 X 表示 NaI 的含量。作为对照，用 30:30:30 的比例加入碘仿而不是 NaI，得到的材料表示为 I30。对物理化学性质（流动性、薄膜厚度、射线不透性、粘度、吸水率、溶解度和离子释放）和生物学性质（细胞毒性、TRAP、ARS 和破骨细胞标志物分析）进行了测定。D30 中的碘、钠和钙离子释放量和 pH 值均高于 I30，而 I30 中检测到的未知提取分子最多。在细胞活力试验中，除 100% D30 组外，所有组均无细胞毒性。在 50%无毒提取物中，D30 组与 I30 组相比，破骨细胞形成减少。综上所述，与碘仿相比，含 NaI 的材料具有足够的物理化学性质和较低的破骨细胞形成。因此，在进一步的（临床前）研究后，含 NaI 的材料可能可替代碘仿作为根管填充材料。
