Chakravarthy U, McCormick D, Maguire C J, Archer D B
Eye (Lond). 1987;1 ( Pt 1):126-35. doi: 10.1038/eye.1987.19.
Standard perforating injuries were created in the right eye of 30 rabbits. Twenty of these had the site of injury irradiated using the radioactive ophthalmic 60Cobalt applicator. Vitreo-retinal membranes obtained from non-irradiated and irradiated eyes were propagated in vitro. The morphology and viability of the cells that grew as a monolayer was studied using phase, light and electron microscopy. The proportions of the different cell types that constituted the monolayer was determined using immunofluorescent staining techniques. Non-irradiated membranes elaborated an abundant outgrowth of healthy cells that were predominantly fibroblasts. Irradiated membranes developed a sparse outgrowth of cells with vacuolated cytoplasm and pyknotic nuclei indicating cell destruction. The majority of the surviving cells were glial, with fibroblasts and retinal pigment epithelial cells forming the remainder.
在30只兔子的右眼造成标准的穿孔伤。其中20只兔子受伤部位使用放射性眼科钴60敷贴器进行照射。从未照射和照射过的眼睛获取玻璃体视网膜膜并在体外进行增殖培养。使用相差显微镜、光学显微镜和电子显微镜研究形成单层生长的细胞的形态和活力。使用免疫荧光染色技术确定构成单层的不同细胞类型的比例。未照射的膜形成大量健康细胞的生长，这些细胞主要是成纤维细胞。照射过的膜形成细胞生长稀疏，细胞质有空泡且细胞核固缩，表明细胞遭到破坏。存活的细胞大多数是神经胶质细胞，其余为成纤维细胞和视网膜色素上皮细胞。
