Schildhaus Hans-Ulrich, Steindor Mathis, Kölsch Bernd, Herold Thomas, Buer Jan, Kehrmann Jan
Institute of Pathology, University Hospital Essen, University of Duisburg-Essen, 45147 Essen, Germany.
Department of Pediatric Pulmonology and Sleep Medicine, Children's Hospital, University of Duisburg-Essen, 45147 Essen, Germany.
J Clin Med. 2022 Apr 25;11(9):2404. doi: 10.3390/jcm11092404.
is the single infectious agent responsible for most deaths worldwide outside of pandemics. Diseases due to non-tuberculous mycobacteria (NTM) are increasing in many regions of the world. The two molecular assays GenoType CM direct (GTCMd) (Bruker, Billerica, MA, USA) and VisionArray Myco (VAM) (ZytoVision, Bremerhaven, Germany) are based on the DNA/DNA hybridization technique, and allow for the identification of tuberculous and the most clinically relevant non-tuberculous mycobacterial species from clinical specimens. We evaluated the performance of both assays for the identification of mycobacteria from 65 clinical specimens of 65 patients and compared it with the results of conventional culture. Based on conventional culture that recovered 37 mycobacterial isolates including 11 tuberculous and 26 NTM isolates, sensitivity, specificity, positive predictive value and negative predictive value were 89.2%, 81.5%, 86.8% and 84.6% for GTCMd and 73.0%, 96.3%, 96.4% and 72.2% for VAM. Additionally, GTCMd identified mycobacteria from five and VAM from one culture-negative sample. Both assays identified a mycobacterium in one sample overgrown by other microorganisms. Two subsp. isolates grown from culture were identified as by GTCMd assay. In conclusion, both assays improve the rapid identification of mycobacteria directly from clinical specimens.
是全球除大流行外导致大多数死亡的单一传染源。在世界许多地区，非结核分枝杆菌（NTM）引起的疾病正在增加。两种分子检测方法，即GenoType CM direct（GTCMd）（美国马萨诸塞州比勒里卡的布鲁克公司）和VisionArray Myco（VAM）（德国不来梅港的ZytoVision公司），基于DNA/DNA杂交技术，可从临床标本中鉴定结核分枝杆菌和临床上最相关的非结核分枝杆菌菌种。我们评估了这两种检测方法从65例患者的65份临床标本中鉴定分枝杆菌的性能，并将其与传统培养结果进行比较。基于传统培养法分离出37株分枝杆菌，其中包括11株结核分枝杆菌和26株NTM菌株，GTCMd的敏感性、特异性、阳性预测值和阴性预测值分别为89.2%、81.5%、86.8%和84.6%，VAM的分别为73.0%、96.3%、96.4%和72.2%。此外，GTCMd从5份培养阴性样本中鉴定出分枝杆菌，VAM从1份培养阴性样本中鉴定出分枝杆菌。两种检测方法都在一个被其他微生物过度生长的样本中鉴定出一种分枝杆菌。通过GTCMd检测，从培养物中生长的两株亚种分离株被鉴定为 。总之，这两种检测方法都提高了直接从临床标本中快速鉴定分枝杆菌的能力。
