Chen Jianlei, Tan Liju, Cui Zhengguo, Qu Keming, Wang Jiangtao
Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.
Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, Ocean University of China, Qingdao 266100, China.
Polymers (Basel). 2022 Apr 29;14(9):1839. doi: 10.3390/polym14091839.
In this study, a novel sample pretreatment strategy of solid-phase microextraction using graphene oxide molecularly imprinted polymers as adsorbents coupled with high-performance liquid chromatography was developed to detect norfloxacin in the marine environment. As a carrier, the imprinted polymers were synthesized by precipitation polymerization with graphene oxide. Compared with graphene oxide non-imprinted polymers, the graphene oxide molecularly imprinted polymers exhibited higher adsorption capacity towards norfloxacin. The synthesized polymeric materials were packed into a molecularly imprinted solid-phase microextraction cartridge, and critical parameters affecting the extraction process were optimized. Under the optimized molecular imprinted solid-phase microextraction condition, the proposed method was applied to the analysis of norfloxacin for seawater and fish with satisfactory recovery (90.1-102.7%) and low relative standard deviation (2.06-5.29%, n = 3). The limit of detection was 0.15 μg L and 0.10 μg kg for seawater and fish, respectively. The study revealed that the proposed molecularly imprinted solid-phase microextraction represents an attractive sample pretreatment strategy for the analysis of norfloxacin in the marine environment.
在本研究中，开发了一种以氧化石墨烯分子印迹聚合物为吸附剂的新型固相微萃取样品预处理策略，并结合高效液相色谱法用于检测海洋环境中的诺氟沙星。以氧化石墨烯为载体，通过沉淀聚合法合成印迹聚合物。与氧化石墨烯非印迹聚合物相比，氧化石墨烯分子印迹聚合物对诺氟沙星表现出更高的吸附容量。将合成的聚合材料填充到分子印迹固相微萃取柱中，并对影响萃取过程的关键参数进行了优化。在优化的分子印迹固相微萃取条件下，该方法应用于海水和鱼肉中诺氟沙星的分析，回收率令人满意（90.1 - 102.7%），相对标准偏差较低（2.06 - 5.29%，n = 3）。海水和鱼肉的检测限分别为0.15 μg/L和0.10 μg/kg。该研究表明，所提出的分子印迹固相微萃取是一种用于分析海洋环境中诺氟沙星的有吸引力的样品预处理策略。
