使用分子印迹硅胶吸附剂结合高效液相色谱法从海洋沉积物和海水样品中选择性萃取和检测诺氟沙星。
Selective extraction and detection of norfloxacin from marine sediment and seawater samples using molecularly imprinted silica sorbents coupled with HPLC.
作者信息
Qin Dan, Zhao Min, Wang Jiangtao, Lian Ziru
机构信息
Marine College, Shandong University, Weihai, 264209, China.
Key Laboratory of Marine Chemistry Theory and Technology, Ministry of Education, College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao, 266100, China.
出版信息
Mar Pollut Bull. 2020 Jan;150:110677. doi: 10.1016/j.marpolbul.2019.110677. Epub 2019 Oct 26.
A simple, sensitive and effective method was developed and validated for selective adsorption and quantitation of norfloxacin (NFX) from marine sediments and seawater samples using the novel molecularly imprinted silica polymers as sorbents followed by high-performance liquid chromatographic analysis with diode array detection (HPLC-DAD). The imprinted particles were characterized by different techniques and the molecular recognition for NFX was also investigated. Several parameters affecting the extraction efficiency of molecularly imprinted solid-phase extraction (MISPE) process were optimized. Three sediment and seawater samples from Weihai bay in China were analyzed and the results show that satisfactory recoveries (77.2-98.7%) were achieved in seawater samples with low relative standard deviation (RSD) values less than 6.89% (n = 3), and the recoveries in sediments were in the range of 75.5-91.7%. The limits of detection are respectivley 2 μg L in seawater samples and 5 μg kg in sediments.
开发并验证了一种简单、灵敏且有效的方法，该方法使用新型分子印迹硅胶聚合物作为吸附剂，从海洋沉积物和海水样品中选择性吸附和定量诺氟沙星（NFX），随后采用带二极管阵列检测的高效液相色谱法（HPLC-DAD）进行分析。通过不同技术对印迹颗粒进行了表征，并研究了其对NFX的分子识别。对影响分子印迹固相萃取（MISPE）过程萃取效率的几个参数进行了优化。分析了来自中国威海湾的三个沉积物和海水样品，结果表明，海水样品的回收率令人满意（77.2-98.7%），相对标准偏差（RSD）值较低，小于6.89%（n = 3），沉积物中的回收率在75.5-91.7%范围内。海水样品和沉积物中的检测限分别为2 μg/L和5 μg/kg。
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