Zhou Zhiwen, Li Jiajia, Zhu Changan, Jing Beiyu, Shi Kai, Yu Jingquan, Hu Zhangjian
Department of Horticulture, Zhejiang University, Hangzhou 310058, China.
Shandong (Linyi) Institute of Modern Agriculture, Zhejiang University, Linyi 276000, China.
Plants (Basel). 2022 Apr 26;11(9):1172. doi: 10.3390/plants11091172.
Due to global warming, high-temperature stress has become a major threat to plant growth and development, which causes a severe challenge to food security worldwide. Therefore, it is necessary to explore the plant bioactive molecules, which could be a promising approach to strengthening plant thermotolerance. Rosmarinic acid (RA) serves as a plant-derived phenolic compound and has beneficial and health-promoting effects for human beings. However, the involvement of RA in plant stress response and the underlying molecular mechanism was largely unknown. In this study, we found that exogenous RA application conferred improved thermotolerance in tomatoes. The transcript abundance and the enzyme activity of enzymatic antioxidants, such as ascorbate peroxidase (APX), catalase (CAT), glutathione reductase (GR), and dehydroascorbate reductase (DHAR), were further promoted by RA treatment in tomato plants subjected to high-temperature stress. Moreover, RA activated the antioxidant system and modulated the cellular redox homeostasis also associated with the redox status of nonenzymatic glutathione and ascorbic acid. The results of RNA-seq data showed that transcriptional regulation was involved in RA-mediated thermotolerance. Consistently, the gene expression of several high temperature-responsive transcription factors like , and WRKY family genes were substantially induced by RA treatment, which potentially contributed to the induction of heat shock proteins (HSPs). Overall, these findings not only gave a direct link between RA and plant thermotolerance but also provided an attractive approach to protecting crop plants from high-temperature damage in a global warming future.
由于全球变暖，高温胁迫已成为植物生长发育的主要威胁，这对全球粮食安全构成了严峻挑战。因此，有必要探索植物生物活性分子，这可能是增强植物耐热性的一种有前景的方法。迷迭香酸（RA）是一种植物源酚类化合物，对人类具有有益和促进健康的作用。然而，RA在植物应激反应中的作用及其潜在分子机制在很大程度上尚不清楚。在本研究中，我们发现外源施用RA可提高番茄的耐热性。在遭受高温胁迫的番茄植株中，RA处理进一步促进了抗氧化酶如抗坏血酸过氧化物酶（APX）、过氧化氢酶（CAT）、谷胱甘肽还原酶（GR）和脱氢抗坏血酸还原酶（DHAR）的转录丰度和酶活性。此外，RA激活了抗氧化系统并调节了细胞氧化还原稳态，这也与非酶促谷胱甘肽和抗坏血酸的氧化还原状态有关。RNA测序数据结果表明，转录调控参与了RA介导的耐热性。一致地，RA处理显著诱导了几个高温响应转录因子如 以及WRKY家族基因的基因表达，这可能有助于热休克蛋白（HSPs）的诱导。总体而言，这些发现不仅建立了RA与植物耐热性之间的直接联系，还提供了一种在全球变暖的未来保护作物免受高温损害的有吸引力的方法。
