Samaras Anastasios, Kamou Nathalie, Tzelepis Georgios, Karamanoli Katerina, Menkissoglu-Spiroudi Urania, Karaoglanidis George S
Laboratory of Plant Pathology, School of Agriculture, Faculty of Agriculture, Forestry and Natural Environment, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
Pesticide Science Laboratory, School of Agriculture, Faculty of Agriculture, Forestry and Natural Environment, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece.
Plants (Basel). 2022 Apr 30;11(9):1218. doi: 10.3390/plants11091218.
MBI600 is a commercialized plant growth-promoting bacterial species used as a biocontrol agent in many crops, controlling various plant pathogens via direct or indirect mechanisms. In the present study, a detailed transcriptomic analysis of cucumber roots upon response to the MBI600 strain is provided. Differentially expressed genes (DEGs) analysis showed altered gene expression in more than 1000 genes at 24 and 48 h post-application of MBI600. MBI600 induces genes involved in ISR and SAR signaling. In addition, genes involved in phytohormone production and nutrient availability showed an upregulation pattern, justifying the plant growth promotion. Biocontrol ability of MBI600 seems also to be related to the activation of defense-related genes, such as peroxidase, endo-1,3(4)-beta-glucanase, PR-4, and thaumatin-like. Moreover, KEGG enriched results showed that differentially expressed genes were classified into biocontrol-related pathways. To further investigate the plant's response to the presence of PGPR, a profile of polar metabolites of cucumber treated with MBI600 was performed and compared to that of untreated plants. The results of the current study gave insights into the mechanisms deployed by this biocontrol agent to promote plant resistance, helping to understand the molecular interactions in this system.
MBI600是一种商业化的促进植物生长的细菌物种，在许多作物中用作生物防治剂，通过直接或间接机制控制各种植物病原体。在本研究中，提供了黄瓜根对MBI600菌株反应的详细转录组分析。差异表达基因（DEG）分析表明，在施用MBI600后24小时和48小时，1000多个基因的表达发生了变化。MBI600诱导参与诱导系统抗性（ISR）和系统获得性抗性（SAR）信号传导的基因。此外，参与植物激素产生和养分有效性的基因呈现上调模式，这证明了其对植物生长的促进作用。MBI600的生物防治能力似乎也与防御相关基因的激活有关，如过氧化物酶、内切-1,3(4)-β-葡聚糖酶、PR-4和类thaumatin蛋白。此外，京都基因与基因组百科全书（KEGG）富集结果表明，差异表达基因被归类为与生物防治相关的途径。为了进一步研究植物对植物根际促生细菌（PGPR）存在的反应，对用MBI600处理的黄瓜的极性代谢物谱进行了分析，并与未处理植物的谱进行了比较。本研究结果深入了解了这种生物防治剂促进植物抗性的机制，有助于理解该系统中的分子相互作用。
