采用多孔石墨碳液相色谱-轨道阱串联质谱法分析二糖至六糖半乳糖低聚糖的谱多样性。
Profile diversity of galacto-oligosaccharides from disaccharides to hexasaccharides by porous graphitic carbon liquid chromatography-orbitrap tandem mass spectrometry.
机构信息
Graduate Institute of Food Science and Technology, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei 10617, Taiwan, ROC.
Graduate Institute of Food Science and Technology, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei 10617, Taiwan, ROC.
出版信息
Food Chem. 2022 Oct 1;390:133151. doi: 10.1016/j.foodchem.2022.133151. Epub 2022 May 6.
Galacto-oligosaccharides (GOS) are important prebiotic supplements for commercial nutraceutical food. The prebiotic efficacy of functional GOS is dependent on their chemical profile. Screening potential markers aids specifications and quality control of GOS materials. However, profiling analysis of GOS with a degree of polymerization (DP) ≥ 4 is still challenging. This study presents a porous graphitic carbon liquid chromatography-orbitrap tandem mass spectrometry-based method that characterized 58 linear and 10 branched GOS and detected 59 non-reducing GOS from DP2 to DP6. The results indicated that 15 major group components with DP2-DP5 accounted for more than 65% of total GOS content in GOS samples, while non-reducing GOS components accounted for only 2.8-7.6%. Substantial variations in components occurred in samples from different batches and sources. Structural and constitutive diversity were dominated by DP3-DP5. This method can help control the quality of GOS products and be used to investigate the structural and prebiotic-efficacy relationships.
半乳糖寡糖（GOS）是商业营养食品的重要益生元补充剂。功能性 GOS 的益生元功效取决于其化学特征。筛选潜在标志物有助于 GOS 材料的规范和质量控制。然而，聚合度（DP）≥4 的 GOS 的分析仍具有挑战性。本研究提出了一种基于多孔石墨碳液相色谱-轨道阱串联质谱的方法，可对 58 种线性和 10 种支链 GOS 进行特征分析，并检测到 DP2 到 DP6 的 59 种非还原 GOS。结果表明，DP2-DP5 的 15 种主要组分组分占 GOS 样品中总 GOS 含量的 65%以上，而非还原 GOS 组分仅占 2.8-7.6%。不同批次和来源的样品中组分存在很大差异。结构和组成多样性主要由 DP3-DP5 决定。该方法有助于控制 GOS 产品的质量，并可用于研究结构和益生元功效关系。
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