College of Chemistry, Beijing University of Chemical Technology, Beijing 100029, China.
Waters Technology (Beijing) Co., Ltd., Jinghai Industrial Park, 156 Jinghai 4th Road, Beijing Economic-Technological Development Area, Beijing 100076, China.
J Agric Food Chem. 2024 Apr 10;72(14):7980-7990. doi: 10.1021/acs.jafc.4c00810. Epub 2024 Apr 2.
Prebiotic oligosaccharides have attracted immense interest in the infant formula (IF) industry due to their unique health benefits for infants. There is a need for the reasonable supplementation of prebiotics in premium IF products. Herein, we characterized the profile of galacto-oligosaccharides (GOS) in human milk (HM) and IF using ultrahigh-performance liquid chromatography-cyclic ion mobility-mass spectrometry (UPLC-cIM-MS) technique. Additionally, we further performed a targeted quantitative analysis of five essential HM oligosaccharides (HMOs) in HM ( = 196), IF ( = 50), and raw milk of IF ( = 10) by the high-sensitivity UPLC-MS/MS method. HM exhibited a more abundant and variable HMO composition (1183.19 to 2892.91 mg/L) than IF (32.91 to 56.31 mg/L), whereas IF contained extra GOS species and non-negligible endogenous 3'-sialyllactose. This also facilitated the discovery of secretor features within the Chinese population. Our study illustrated the real disparity in the prebiotic glycome between HM and IF and provided crucial reference for formula improvement.
由于其对婴儿独特的健康益处，益生元低聚糖在婴儿配方食品（IF）行业引起了极大的关注。需要在优质 IF 产品中合理补充益生元。在此，我们使用超高效液相色谱-循环离子淌度-质谱（UPLC-cIM-MS）技术对人乳（HM）和 IF 中的半乳糖低聚糖（GOS）谱进行了表征。此外，我们还通过高灵敏度 UPLC-MS/MS 方法对 HM（= 196）、IF（= 50）和 IF 原料乳（= 10）中的五种必需 HM 低聚糖（HMO）进行了靶向定量分析。与 IF（32.91 至 56.31 毫克/升）相比，HM 表现出更丰富和更多变的 HMO 组成（1183.19 至 2892.91 毫克/升），而 IF 则含有额外的 GOS 种类和不可忽视的内源性 3'-唾液酸乳糖。这也有助于发现中国人群中的分泌特征。我们的研究说明了 HM 和 IF 之间在益生元糖组学上的真实差异，并为配方改进提供了重要参考。
