Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
Exp Cell Res. 2022 Aug 1;417(1):113205. doi: 10.1016/j.yexcr.2022.113205. Epub 2022 May 11.
The human endometrium is a dynamic tissue that undergoes cyclic changes in response to sex steroid hormones to provide a receptive status for embryo implantation. Disruptions in this behavior may lead to implantation failure and infertility; therefore, it is essential to develop an appropriate in vitro model to study endometrial changes in response to sex hormones. In this regard, the first choice would be human endometrial cells isolated from biopsies that could be used as monolayer cell sheets or to generate endometrial organoids. However, the need for fresh samples and short-time viability of harvested endometrial biopsy limits these approaches. In order to overcome these limitations, we sought to develop an efficient, simple, robust and reproducible method to cryopreserve human endometrial biopsies that could be stored and/or shipped frozen and later thawed to generate endometrial organoids and endometrial stromal cells (EnSCs). These cryopreserved biopsies could be thawed and used to generate simple endometrial organoids or organoids for co-culture with matched stromal cells that are functionally responsive to sex hormones as similar as the organoids generated from fresh biopsy. An optimal endometrial tissue cryopreservation method would allow the possibility for endometrial tissue biobanking to enable future organoid generation from both healthy tissues and pathological conditions, and open new venues for generate endometrial assembloids, consisting of epithelial organoids and primary stromal cells.
人类子宫内膜是一种动态组织，它会对性激素产生周期性变化，以提供胚胎植入的接受状态。这种行为的中断可能导致着床失败和不孕；因此，开发一种适当的体外模型来研究性激素对子宫内膜的变化是至关重要的。在这方面，首选的方法是从活检中分离出人子宫内膜细胞，可以将其用作单层细胞片或生成子宫内膜类器官。然而，对新鲜样本的需求和收获的子宫内膜活检的短时间存活率限制了这些方法的应用。为了克服这些限制，我们试图开发一种高效、简单、稳健和可重复的方法来冷冻保存人子宫内膜活检，可以将其冷冻储存和/或运输，然后解冻以生成子宫内膜类器官和子宫内膜基质细胞（EnSCs）。这些冷冻保存的活检可以解冻并用于生成简单的子宫内膜类器官，或与匹配的基质细胞共培养的类器官，这些类器官对性激素的反应与从新鲜活检生成的类器官相似。一种最佳的子宫内膜组织冷冻保存方法将允许进行子宫内膜组织生物库存储，以便将来从健康组织和病理条件下生成类器官，并为生成由上皮类器官和原代基质细胞组成的子宫内膜组合体开辟新途径。
