Division of Biomedical Sciences, Warwick Medical School, University of Warwick, Clinical Sciences Building, University Hospital Coventry, Coventry, UK.
Centre for Reproductive Medicine, University Hospitals Coventry and Warwickshire NHS Trust, Coventry, UK.
Methods Mol Biol. 2024;2767:63-74. doi: 10.1007/7651_2023_495.
Understanding the process of human embryo implantation is impeded by the inability to study this phenomenon in vivo, thus limiting opportunities to gain knowledge to in vitro modeling. Previous models have relied on monolayer co-cultures, which do not replicate the complexity of endometrial tissue. Here, we detail the establishment of three-dimensional endometrial assembloids, comprising gland-like epithelial organoids in a stromal matrix. Endometrial assembloids mimic endometrial tissue structure more faithfully and can be used to study human embryo-endometrial interactions. Co-cultures of human embryos and endometrial assembloids will enhance our fundamental understanding of these processes as well as allowing us to study the mechanisms of persistent reproductive failure.
理解人类胚胎着床的过程受到在体内研究这一现象的能力的阻碍，从而限制了从体外模型中获得知识的机会。以前的模型依赖于单层共培养，不能复制子宫内膜组织的复杂性。在这里，我们详细介绍了三维子宫内膜组装体的建立，包括在基质中的腺体样上皮类器官。子宫内膜组装体更真实地模拟了子宫内膜组织的结构，可用于研究人类胚胎-子宫内膜的相互作用。人类胚胎和子宫内膜组装体的共培养将增强我们对这些过程的基本理解，并使我们能够研究持续性生殖失败的机制。
