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甘氨酸甜菜碱调节六价铬诱导的形态生理和生化反应,减轻鹰嘴豆(Cicer arietinum L.)品种的铬毒性。

Glycine betaine modulates chromium (VI)-induced morpho-physiological and biochemical responses to mitigate chromium toxicity in chickpea (Cicer arietinum L.) cultivars.

机构信息

Department of Botany, Meerut College, Meerut, 250001, India.

Division of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India.

出版信息

Sci Rep. 2022 May 14;12(1):8005. doi: 10.1038/s41598-022-11869-3.

Abstract

Chromium (Cr) accumulation in crops reduces yield. Here, we grew two chickpea cultivars, Pusa 2085 (Cr-tolerant) and Pusa Green 112 (Cr-sensitive), in hydroponic and pot conditions under different Cr treatments: 0 and 120 µM Cr and 120 µM Cr + 100 mM glycine betaine (GB). For plants grown in the hydroponic media, we evaluated root morphological attributes and plasma membrane integrity via Evans blue uptake. We also estimated H-ATPase activity in the roots and leaves of both cultivars. Plants in pots under conditions similar to those of the hydroponic setup were used to measure growth traits, oxidative stress, chlorophyll contents, enzymatic activities, proline levels, and nutrient elements at the seedling stage. Traits such as Cr uptake in different plant parts after 42 days and grain yield after 140 days of growth were also evaluated. In both cultivars, plant growth traits, chlorophyll contents, enzymatic activities, nutrient contents, and grain yield were significantly reduced under Cr stress, whereas oxidative stress and proline levels were increased compared to the control levels. Further, Cr uptake was remarkably decreased in the roots and leaves of Cr-tolerant than in Cr-sensitive cultivars. Application of GB led to improved root growth and morpho-physiological attributes and reduced oxidative stress along with reduced loss in plasma membrane integrity and subsequently increase in H-ATPase activity. An increment in these parameters shows that the exogenous application of GB improves the Cr stress tolerance in chickpea plants.

摘要

铬(Cr)在作物中的积累会降低产量。在这里,我们在水培和盆栽条件下用两种鹰嘴豆品种,Pusa 2085(耐铬)和 Pusa Green 112(敏感)进行了不同 Cr 处理的实验:0 和 120µM Cr 以及 120µM Cr+100mM 甘氨酸甜菜碱(GB)。对于在水培介质中生长的植物,我们通过 Evans 蓝摄取评估了根形态特征和质膜完整性。我们还估计了两个品种根和叶中的 H-ATP 酶活性。在类似于水培设置的条件下在盆栽中的植物用于测量幼苗期的生长特性、氧化应激、叶绿素含量、酶活性、脯氨酸水平和营养元素。还评估了 42 天后不同植物部分的 Cr 吸收和 140 天后的籽粒产量等特性。在两个品种中,与对照相比,Cr 胁迫下植物生长特性、叶绿素含量、酶活性、营养含量和籽粒产量均显著降低,而氧化应激和脯氨酸水平增加。此外,Cr 耐受品种的根和叶中 Cr 吸收明显低于敏感品种。GB 的应用导致根生长和形态生理特性的改善,氧化应激减少,质膜完整性损失减少,随后 H-ATP 酶活性增加。这些参数的增加表明,外源 GB 的应用提高了鹰嘴豆植物对 Cr 胁迫的耐受性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/736e/9107477/41a2de733ad7/41598_2022_11869_Fig1_HTML.jpg

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