Bueno Thaís Silveira, Loiko Márcia Regina, Vidaletti Marina Roth, de Oliveira Júlia Alves, Fetzner Tiago, Cerva Cristine, de Moraes Lucas Brunelli, De Carli Silvia, Siqueira Franciele Maboni, Rodrigues Rogério Oliveira, Coppola Mario de Menezes, Callegari-Jacques Sidia Maria, Mayer Fabiana Quoos
Centro de Pesquisa em Saúde Animal, Departamento de Diagnóstico e Pesquisa Agropecuária, Instituto de Pesquisas Veterinárias DesidérioFinamor, Secretaria de Agricultura, Pecuária e Desenvolvimento Rural, Eldorado do Sul, Brazil.
Universidade Feevale, Novo Hamburgo, Brazil.
Zoonoses Public Health. 2022 Sep;69(6):682-693. doi: 10.1111/zph.12957. Epub 2022 May 15.
Bacterial resistance is a public and one health problem. Free-living birds can be reservoirs of multidrug-resistant bacteria and resistance genes. This study aimed to characterize the antimicrobial resistance of Escherichia coli isolated from free-living urban pigeons (Columba livia) in South Brazil. Ninety-two animals were sampled, and one isolate was obtained from each one. The isolates were characterized, and the antimicrobial resistance profile and beta-lactam and colistin resistance genes were investigated. The isolates were classified as phylogroups B1 (35%), B2 (33%), A (16%) and D (16%), and 14% of the strains had the eae virulence gene. All isolates were resistant to at least one antimicrobial, and 63% of them were multidrug-resistant. Geographical location where the pigeons were captured and presence of the eae gene were associated with multidrug resistance. bla and mcr-1 genes were detected in one and two isolates, respectively. This is the first report of these genes in E. coli of pigeons. The bla -positive isolate was classified as Shiga toxin-producing E. coli, and the isolates with mcr-1 were classified as Enterohaemorrhagic E. coli and Enteropathogenic E. coli, which raise additional concerns related to public health since these are zoonotic pathotypes. The results reveal that pigeons carry multidrug-resistant pathogenic E. coli, which may interest public health. Nonetheless, further studies on whether these animals are sources of contamination for humans must be performed to understand their role in spreading antimicrobial resistance.
细菌耐药性是一个公共卫生问题，也是一个“同一健康”问题。自由生活的鸟类可能是多重耐药细菌和耐药基因的储存宿主。本研究旨在对从巴西南部城市自由生活的家鸽（Columba livia）中分离出的大肠杆菌的抗菌药物耐药性进行表征。对92只动物进行了采样，每只动物获得一个分离株。对分离株进行了表征，并研究了抗菌药物耐药谱以及β-内酰胺和黏菌素耐药基因。分离株被分为B1型（35%）、B2型（33%）、A型（16%）和D型（16%），14%的菌株具有eae毒力基因。所有分离株均对至少一种抗菌药物耐药，其中63%为多重耐药。鸽子被捕地点和eae基因的存在与多重耐药性相关。分别在1株和2株分离株中检测到bla和mcr-1基因。这是这些基因在家鸽大肠杆菌中的首次报道。bla阳性分离株被分类为产志贺毒素大肠杆菌，mcr-1阳性分离株被分类为肠出血性大肠杆菌和肠致病性大肠杆菌，由于这些是人畜共患病原体类型，这引发了与公共卫生相关的更多担忧。结果表明，鸽子携带多重耐药的致病性大肠杆菌，这可能引起公共卫生的关注。尽管如此，仍需进一步研究这些动物是否为人类的污染源，以了解它们在传播抗菌药物耐药性中的作用。
