College of Health Sciences-QU Health, Qatar University, Doha, Qatar.
Biomedical Research Center, Qatar University, Doha, Qatar.
Microb Drug Resist. 2020 Apr;26(4):420-427. doi: 10.1089/mdr.2019.0402. Epub 2020 Apr 1.
This study aims at evaluating the phenotypic and genotypic antimicrobial resistance (AMR) patterns of 18 clinically relevant antibiotics in food animals in Qatar. Fecal samples from camels, cattle, and pigeons (300) were collected from different slaughterhouses and farms. isolates were recovered on selective media, confirmed biochemically, and tested for antibiotic susceptibility using a disk diffusion assay. Any isolate that showed resistance to colistin was confirmed using the E-test and polymerase chain reaction for genes. Overall, a total of 88.7% ( = 266/300) recovery rate was achieved from all samples. Resistance to at least one antibiotic was recorded in 70.7% of pigeons, 37.2% of cattle, and only 20.8% of camel samples. Multidrug resistance (MDR) was highest in isolates from pigeons, 50% ( = 44). Moreover, trimethoprim/sulfamethoxazole (an antibiotic used to treat a variety of bacterial infections) resistance was present in 22.2% ( = 59) of all isolates. Only one isolate from a pigeon showed resistance to colistin ( gene encoded), a drug of last resort in human medicine against gram-negative bacterial infection. We previously reported high multidrug resistance of in chickens, with significant resistance to colistin. We observed a lower AMR profile in ruminants. The high resistance profile observed in pigeons (70.7%), including high multidrug resistance (50%), is alarming as these animals could rapidly disseminate resistant bacteria to various locations. Continuous monitoring of AMR in livestock in Qatar is necessary toward introducing an antimicrobial stewardship program and control of antibiotic usage in the veterinary sector.
本研究旨在评估卡塔尔食品动物中 18 种临床相关抗生素的表型和基因型抗菌药物耐药（AMR）模式。从不同的屠宰场和农场采集了 300 份骆驼、牛和鸽子的粪便样本。在选择性培养基上回收分离物，通过生化方法确认，并使用纸片扩散法测试抗生素敏感性。任何对粘菌素表现出耐药性的分离物都使用 E 试验和聚合酶链反应（PCR）对 基因进行确认。总体而言，从所有样本中获得了 88.7%（=266/300）的总回收率。在 70.7%的鸽子、37.2%的牛和仅 20.8%的骆驼样本中记录到至少对一种抗生素的耐药性。鸽子分离物的多重耐药性（MDR）最高，为 50%（=44）。此外，所有分离物中 22.2%（=59）对复方磺胺甲噁唑（一种用于治疗多种细菌感染的抗生素）耐药。只有一只来自鸽子的 分离物对粘菌素（基因编码）表现出耐药性，这是人类医学中治疗革兰氏阴性细菌感染的最后手段。我们之前报道过鸡中 存在高多重耐药性，对粘菌素有显著耐药性。我们在反刍动物中观察到较低的 AMR 谱。鸽子（70.7%）中观察到的高耐药性，包括高多重耐药性（50%），令人警惕，因为这些动物可能会迅速将耐药细菌传播到各个地方。有必要对卡塔尔的牲畜进行 AMR 持续监测，以实施抗菌药物管理计划并控制兽医部门的抗生素使用。
