InBio, Food Science Group, Charlottesville, VA 22903, USA.
InBio, Analytics Group, Cardiff, Wales, UK.
Food Chem. 2022 Sep 30;389:132986. doi: 10.1016/j.foodchem.2022.132986. Epub 2022 Apr 18.
The aim was to develop a fluorescent multiplex array for simultaneously measuring regulated food allergens using specific allergen protein molecules from peanut, tree nut, cow's milk, egg, soy, fish, shellfish, sesame, mustard and celery. Microspheres coupled to specific monoclonal antibodies were used for allergen detection, with purified allergens as reference standards.Standard curves for 17 allergens covered a 5-log dynamic range. Intra- and inter-assay acceptance criteria were within 70-130% recovery and a CV of ≤15%. Food reference materials contained high levels of their respective major allergens (2000-175,000 µg/g), Similar high allergen levels were found in 10 selected foods analysed using a 9-plex array. Egg, milk, peanut, hazelnut and walnut allergens were detectable in chocolate bars with incurred allergens at 3, 10, 30, and 100 ppm. The multiplex array is an efficient tool for measuring specific food allergens, with applications for risk assessment and standardization of therapeutic products for food allergy.
本研究旨在开发一种荧光多重阵列，用于同时测量花生、坚果、牛奶、鸡蛋、大豆、鱼类、贝类、芝麻、芥末和芹菜中特定过敏原蛋白分子的调控食物过敏原。使用与特定单克隆抗体偶联的微球进行过敏原检测，以纯化过敏原作为参考标准。17 种过敏原的标准曲线涵盖了 5 个对数动态范围。内和间分析接受标准在 70-130%回收率和 CV ≤15%范围内。食品参考材料含有各自主要过敏原的高水平（2000-175,000 µg/g），使用 9 重阵列分析的 10 种选定食品中也发现了类似的高过敏原水平。在含有 3、10、30 和 100 ppm 过敏原的巧克力棒中可检测到鸡蛋、牛奶、花生、榛子和核桃过敏原。多重阵列是一种用于测量特定食物过敏原的有效工具，可用于食物过敏的风险评估和治疗产品的标准化。
