Shortill Shawn P, Frier Mia S, Conibear Elizabeth
Department of Medical Genetics, University of British Columbia, Vancouver, BC VH6 3N1, Canada; Centre for Molecular Medicine and Therapeutics, British Columbia Children's Hospital Research Institute, University of British Columbia, Vancouver, BC V5Z 4H4, Canada.
Department of Medical Genetics, University of British Columbia, Vancouver, BC VH6 3N1, Canada; Centre for Molecular Medicine and Therapeutics, British Columbia Children's Hospital Research Institute, University of British Columbia, Vancouver, BC V5Z 4H4, Canada.
Curr Opin Cell Biol. 2022 Jun;76:102087. doi: 10.1016/j.ceb.2022.102087. Epub 2022 May 12.
The endolysosomal network consists of highly dynamic membrane-bound compartments that control subcellular degradative and recycling processes. A conserved family of endosomal coat complexes known as SNX-BARs drive the formation of tubular membrane transport carriers for cargo retrieval. Whereas SNX1-related SNX-BARs were previously thought to rely on their association with the retromer complex to recognize cargo, recent work shows this class of SNX-BARs can directly bind and deliver cargo. In this review, we examine the retromer-independent roles of SNX-BAR proteins in yeast and metazoans and explore their functional overlap with endosomal sorting complexes and accessory factors. We also discuss new work that highlights the role of the disordered N-terminal regions of SNX-BARs in complex assembly and function.
内溶酶体网络由高度动态的膜结合区室组成，这些区室控制着亚细胞降解和再循环过程。一类保守的内体包被复合物家族，即分选衔接蛋白- Bin/Amphiphysin/Rvs（SNX - BAR）结构域蛋白，驱动管状膜运输载体的形成以进行货物回收。虽然与分选衔接蛋白1（SNX1）相关的SNX - BAR结构域蛋白以前被认为依赖于它们与回收复合物的结合来识别货物，但最近的研究表明，这类SNX - BAR结构域蛋白可以直接结合并运输货物。在这篇综述中，我们研究了SNX - BAR蛋白在酵母和后生动物中不依赖回收复合物的作用，并探讨了它们与内体分选复合物及辅助因子的功能重叠。我们还讨论了新的研究工作，这些工作突出了SNX - BAR结构域蛋白无序的N端区域在复合物组装和功能中的作用。
