Key Laboratory of Hangzhou City for Ecosystem Protection and Restoration, School of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, Zhejiang, China.
Key Laboratory of Hangzhou City for Ecosystem Protection and Restoration, School of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, Zhejiang, China.
Ecotoxicol Environ Saf. 2022 Jul 1;239:113621. doi: 10.1016/j.ecoenv.2022.113621. Epub 2022 May 12.
Environmental issues associated with the widespread use of agricultural chemicals are being seriously concerned. Of them, toxicological impacts of fungicides in aquatic organisms are often overlooked. Here, soft-shelled turtle (Pelodiscus sinensis) hatchlings were exposed to different concentrations of vinclozolin (0, 5, 50, 500 and 5000 μg/L) for 60 days to investigate the impact of fungicide exposure on their gut microbial composition and diversity. Vinclozolin exposure significantly affected the composition of the gut microbiota in hatchling turtles. Unexpectedly, gut bacterial diversity and richness of vinclozolin-exposed turtles (but not for the 5000 μg/L-exposed group) were relatively higher than control ones. At the phylum level, the abundance of Firmicutes was decreased, while that of Proteobacteria was increased in high-concentration groups. At the genus level, some bacterial genera including Cellulosilyticum, Romboutsia and Clostridium_sensu_stricto, were significantly changed after vinclozolin exposure; and some uniquely observed in high-concentration groups. Gene function predictions showed that genes related to amino acid metabolism were less abundant, while those related to energy metabolism more abundant in high-concentration groups. The prevalence of some pathogens inevitably affected gut health status of vinclozolin-exposed turtles. Such gut microbiota dysbiosis might be potentially linked with hepatic metabolite changes induced by vinclozolin exposure.
与农业化学品广泛使用相关的环境问题正受到严重关注。其中，杀菌剂对水生生物的毒理学影响往往被忽视。在这里，我们将中华鳖（Pelodiscus sinensis）幼鳖暴露于不同浓度的咯菌腈（0、5、50、500 和 5000μg/L）中 60 天，以研究杀菌剂暴露对其肠道微生物组成和多样性的影响。咯菌腈暴露显著影响了幼鳖肠道微生物群落的组成。出乎意料的是，咯菌腈暴露组的肠道细菌多样性和丰富度（但不是 5000μg/L 暴露组）相对高于对照组。在门水平上，厚壁菌门的丰度降低，而变形菌门的丰度在高浓度组中增加。在属水平上，一些细菌属，包括纤维分解菌属、罗姆布茨氏菌属和严格梭菌属，在咯菌腈暴露后发生了显著变化；并且在高浓度组中观察到一些独特的细菌属。基因功能预测表明，与氨基酸代谢相关的基因丰度较低，而与能量代谢相关的基因丰度在高浓度组中较高。一些病原体的流行不可避免地影响了咯菌腈暴露组鳖的肠道健康状况。这种肠道微生物失调可能与咯菌腈暴露诱导的肝代谢物变化有关。
