Peritore Alessio Filippo, Franco Gianluca Antonio, Molinari Francesco, Arangia Alessia, Interdonato Livia, Marino Ylenia, Cuzzocrea Salvatore, Gugliandolo Enrico, Britti Domenico, Crupi Rosalia
Department of Veterinary Science, University of Messina, 98168 Messina, Italy.
Department of of Chemical, Biological, Pharmaceutical and Environmental Science, University of Messina, 98166 Messina, Italy.
Toxics. 2023 May 23;11(6):473. doi: 10.3390/toxics11060473.
(1) Background: Vinclozolin is a popular fungicide used in fruit, ornamental plants, and vegetable crops. It has recently been seen that prolonged exposure to VZN can cause human or animal health damage to various organs, but little is known to date about its cardiovascular effects. In this study, we addressed the chronic effects of VZN on the myocardium and the enzymes involved in the cardiovascular function. (2) Methods: The animals were divided into four groups: group 1 served as the control, group 2 received 1 mg/kg of VZN by gavage, group 3 received 30 mg/kg of VZN by gavage, and group 4 received 100 mg/kg of VZN by gavage, for 30 days. (3) Results: Results showed that 100 mg/kg VZN markedly increased the plasma concentration of cardiac markers (CK-MB, cTnT, ANP, BNP). Moreover, compared to the control group, VZN treatment decreased the activity of SOD, CAT, and GPx, and downregulated the mRNA expression levels of Nrf2. Furthermore, collagen deposition was amplified owing to 100 mg/kg VZN cardiotoxicity. This harmful effect was confirmed by a histological study using hematoxylin and eosin (H&E) and Masson's trichrome staining. (4) Conclusion: Overall, our results proved the cardiotoxicity caused by chronic exposure to VZN.
(1) 背景：乙烯菌核利是一种广泛用于水果、观赏植物和蔬菜作物的杀菌剂。最近发现，长期接触乙烯菌核利会对人类或动物的各个器官造成健康损害，但迄今为止，关于其对心血管系统的影响知之甚少。在本研究中，我们探讨了乙烯菌核利对心肌及心血管功能相关酶的慢性影响。(2) 方法：将动物分为四组：第1组作为对照组，第2组通过灌胃给予1 mg/kg乙烯菌核利，第3组通过灌胃给予30 mg/kg乙烯菌核利，第4组通过灌胃给予100 mg/kg乙烯菌核利，持续30天。(3) 结果：结果显示，100 mg/kg乙烯菌核利显著提高了心脏标志物（肌酸激酶同工酶、心肌肌钙蛋白T、心钠素、脑钠肽）的血浆浓度。此外，与对照组相比，乙烯菌核利处理降低了超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶的活性，并下调了核因子E2相关因子2的mRNA表达水平。此外，由于100 mg/kg乙烯菌核利的心脏毒性，胶原沉积增加。使用苏木精和伊红（H&E）及马松三色染色的组织学研究证实了这种有害影响。(4) 结论：总体而言，我们的结果证明了长期接触乙烯菌核利会导致心脏毒性。
