Development of silk fibroin‑sodium alginate scaffold loaded silk fibroin nanoparticles for hemostasis and cell adhesion.

During wound healing process, it is essential to promote hemostasis and cell adhesion. Herein, we incorporated a scaffold with nanoparticles to improve the hemostatic properties and stimulate cell adhesion. The nanoparticles were prepared by self-assembling of silk fibroin, and the scaffold loaded nanoparticles were synthesized by crosslinking and freeze-drying. Macroscopical images showed that the nanoparticles distributed uniformly and increased the surface roughness of scaffold pore wall. The addition of nanoparticles decreased the pore size, enhanced the compression strength, lowered the degradation rate, and maintained the resilience and water uptake capacity. Compared with pure scaffold, the scaffold loaded nanoparticles revealed higher blood clotting index and promoted platelets adhesion. Furthermore, in vitro tests showed that scaffold loaded nanoparticles exhibited excellent biocompatibility, and stimulation effects on cell proliferation, migration, and adhesion for both L929 cells and HUVECs. Therefore, the scaffold loaded nanoparticles possessed great potential as a wound dressing for efficient hemostasis and subsequent wound healing.