Environment and Biofuel Research Laboratory, Department of Hydro and Renewable Energy (HRED), Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247667, India.
Environment and Biofuel Research Laboratory, Department of Hydro and Renewable Energy (HRED), Indian Institute of Technology Roorkee, Roorkee, Uttarakhand 247667, India.
Bioresour Technol. 2022 Jul;356:127306. doi: 10.1016/j.biortech.2022.127306. Epub 2022 May 12.
The present work aimed to evaluate the mixotrophic growth of Chlorella pyrenoidosa in a microalgal-biofilm reactor (MBR) using waste glycerol as an organic carbon source. The biomass productivity of C. pyrenoidosa (10.14 g m d) under the mixotrophic mode was remarkably higher than that observed during the phototrophic mode (4.16 g m d), under similar incubation conditions. The hydraulic retention time (HRT) of 6 d was found optimal for the higher productivity of microalgae in the MBR. Notably, based on biofuel quality, mixotrophically grown microalgal biomass was noted to have better suitability for biomethane production compared to biodiesel. Besides, up to 98.09, 75.74, and 55.86% removal of phosphate, nitrate, and COD, respectively, was recorded within 6 d under mixotrophic growth. Overall, the present findings magnificently demonstrate the efficient recycling of waste glycerol for higher biomass production coupled with phycoremediation using mixotrophic MBR.
本工作旨在评估微藻-生物膜反应器（MBR）中以废甘油为有机碳源的蛋白核小球藻混合营养生长。在相似的培养条件下，混合营养模式下的 C. pyrenoidosa 生物量生产力（10.14 g·m-3·d-1）明显高于光营养模式（4.16 g·m-3·d-1）。水力停留时间（HRT）为 6 d 时，MBR 中微藻的更高生产力达到最佳。值得注意的是，基于生物燃料质量，与生物柴油相比，混合营养生长的微藻生物质更适合生产生物甲烷。此外，在混合营养生长下，分别在 6 d 内实现了高达 98.09%、75.74%和 55.86%的磷酸盐、硝酸盐和 COD 的去除率。总的来说，本研究结果极好地证明了利用混合营养 MBR 高效回收废甘油以实现更高生物量生产和光修复。
