Department of Wildlife and Ecology, University of Veterinary and Animal Sciences, Lahore, Pakistan.
Department of Botany, University of the Punjab, Lahore, Pakistan.
Chemosphere. 2022 Aug;301:134703. doi: 10.1016/j.chemosphere.2022.134703. Epub 2022 Apr 25.
Microalgae are the important biofuel precursors and their economic cultivation can be boosted under mixotrophic (MT) conditions while employing different industrial wastewaters containing organic carbon. In the current research, the quantitative analysis of microalgal biomass production under MT and photoautotrophic (PT) cultivation conditions both at lab and pilot scales was studied. For the purpose, a pre-identified microalgal species Chlorella sorokiniana was cultivated mixotrophically and photoautotrophically at lab and pilot scales. Artificially prepared wastewater containing 2% (w/v) sugarcane molasses was used for MT cultivation. However, for PT cultivation, atmospheric CO was the only carbon source. After 15 days of aerobic incubation, microalgal biomass was harvested and analyzed for biomass productivity. Cultivation conditions and cultivation scale posed significant and non-significant impact, respectively on biomass productivities. However, biomass productivity was comparatively higher for the biomass raised under MT conditions at lab scale. The recorded values of biomass productivity were 88.75 ± 9.51 and 127.68 ± 7.91 mg L d for the biomass raised at lab scale under PT and MT conditions, respectively. Pilot-scale cultivation depicted biomass productivities as 83.49 ± 7.87 and 124.88 ± 3.76 mg L d under PT and MT conditions, respectively. High biomass production under MT conditions may suggest the elevated production of biofuels from microalgae. Future studies on biomass production while utilizing different industrial wastewaters at pilot scale and in open raceway ponds are needed for viable production of microalgae-based fuels.
微藻是重要的生物燃料前体，在采用含有有机碳的不同工业废水进行混合营养（MT）培养时，可以促进其经济培养。在当前的研究中，研究了在 MT 和光自养（PT）培养条件下在实验室和中试规模下微藻生物量生产的定量分析。为此，在实验室和中试规模下，以预先确定的微藻物种 Chlorella sorokiniana 进行了混合营养和光自养培养。使用人工制备的含有 2%（w/v）甘蔗糖蜜的废水进行 MT 培养。然而，对于 PT 培养，大气 CO 是唯一的碳源。有氧孵育 15 天后，收获微藻生物质并进行生物质生产力分析。培养条件和培养规模分别对生物质生产力产生显著和非显著影响。然而，在实验室规模下 MT 条件下培养的生物质的生物质生产力相对较高。在 PT 和 MT 条件下，在实验室规模下培养的生物质的记录生物质生产力值分别为 88.75±9.51 和 127.68±7.91mg L d。在 PT 和 MT 条件下，中试规模培养的生物质生产力分别为 83.49±7.87 和 124.88±3.76mg L d。MT 条件下高生物质产量可能表明微藻生物燃料的产量增加。需要在中试规模和开放跑道池塘中利用不同的工业废水进行生物量生产的后续研究，以实现微藻基燃料的可行生产。
