State Key Laboratory of Crop Biology, National Research Center for Apple Engineering and Technology, Collaborative Innovation Center of Fruit & Vegetable Quality and Efficient Production, Shandong Agricultural University, Tai'an 271018, Shandong, China.
Shandong Institue of Pomology, Taian, Shandong 271018, China.
Gene. 2022 Jul 20;832:146558. doi: 10.1016/j.gene.2022.146558. Epub 2022 May 13.
Amino acid permeases (AAPs) play important roles in plant amino acid transport and nitrogen metabolism. In this study, we carried a comprehensive analysis for apple genes encoding AAPs using bioinformatics and molecular biology. Eleven MdAAPs were identified by a genome-wide search and comparative genomic analysis revealed relatively conserved gene composition, transmembrane characteristics, and protein structures. Phylogenetic tree construction and analysis of the conserved motifs of MdAAPs and AtAAPs showed that AAPs can be classified into three groups (I, II, and III). We compared the promoters of the identified genes and did gene functional annotation and qRT-PCR and found a relationship between apple AAPs and nitrogen deficiency. The expression profile data implied that MdAAPs exhibit diversified distributions and functions in different tissues.
氨基酸通透酶（AAPs）在植物氨基酸运输和氮代谢中发挥着重要作用。本研究采用生物信息学和分子生物学方法，对苹果中编码 AAPs 的基因进行了全面分析。通过全基因组搜索鉴定了 11 个 MdAAPs，比较基因组分析显示这些基因具有相对保守的基因组成、跨膜特性和蛋白质结构。MdAAPs 和 AtAAPs 的系统发育树构建和保守基序分析表明，AAPs 可以分为三组（I、II 和 III）。我们比较了鉴定基因的启动子，并进行了基因功能注释和 qRT-PCR，发现苹果 AAPs 与氮缺乏有关。表达谱数据表明，MdAAPs 在不同组织中表现出多样化的分布和功能。
