Natural Products Research Center, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, Sichuan, 610041, PR China; Savaid Medical School, University of Chinese Academy of Sciences, Beijing, 100049, PR China.
Natural Products Research Center, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, Sichuan, 610041, PR China.
Anal Chim Acta. 2022 May 29;1209:339816. doi: 10.1016/j.aca.2022.339816. Epub 2022 Apr 8.
Organelles play specific and indispensable roles in cellular processes, and their dysfunction causes a series of diseases. Monitoring their variations is crucial to understanding their roles in physiopathology. Unlike most organelle imaging tools based on chemical probes or proteins, we developed a novel molecular tool for cell organelle imaging based on nucleic acids. Real-time imaging of mitochondria and nucleus in living cells was realized easily through using RNA-based tools, which are composed of Organelle-targeted RNA Sequence and Fluorogen-activating RNA aptamer. This genetically encoded Organelle-targeted RNA imaging system provides a powerful platform for measuring the properties of organelles and intracellular structures in living cells, due to its nontoxicity, low fluorescence background, ease of construction, and modular properties.
细胞器在细胞过程中发挥特定且不可或缺的作用，其功能障碍会导致一系列疾病。监测它们的变化对于了解它们在生理病理学中的作用至关重要。与大多数基于化学探针或蛋白质的细胞器成像工具不同，我们基于核酸开发了一种新型的细胞器成像分子工具。通过使用由细胞器靶向 RNA 序列和荧光基团激活 RNA 适体组成的 RNA 工具，可以轻松实现活细胞中线粒体和细胞核的实时成像。由于其无毒、荧光背景低、易于构建和模块化等特性，这种遗传编码的细胞器靶向 RNA 成像系统为测量活细胞中细胞器和细胞内结构的特性提供了一个强大的平台。
