Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, Moscow 117997, Russia.
Institute of Biochemical Technology and Nanotechnology, RUDN University, Miklukho-Maklaya 6, Moscow 117198, Russia.
Int J Mol Sci. 2024 Sep 27;25(19):10448. doi: 10.3390/ijms251910448.
In the present study, we demonstrated that the introduction of a 1,4-diethyl-1,2,3,4-tetrahydroquinoxalin moiety into the arylidene part of GFP chromophore-derived compounds results in the formation of environment-sensitive fluorogens. The rationally designed and synthesized compounds exhibit remarkable solvent- and pH-dependence in fluorescence intensity. The solvent-dependent variation in fluorescence quantum yield makes it possible to use some of the proposed compounds as polarity sensors suitable for selective endoplasmic reticulum fluorescent labeling in living cells. Moreover, the pH-dependent emission intensity variation of other fluorogens makes them selective fluorescent labels for the lysosomes in living cells.
在本研究中，我们证明了在 GFP 发色团衍生化合物的芳基亚基部分引入 1,4-二乙基-1,2,3,4-四氢喹喔啉基可以形成对环境敏感的荧光团。经过合理设计和合成的化合物在荧光强度上表现出显著的溶剂和 pH 值依赖性。荧光量子产率的溶剂依赖性变化使得一些所提出的化合物有可能成为适用于活细胞中选择性内质网荧光标记的极性传感器。此外，其他荧光团的 pH 值依赖性发射强度变化使它们成为活细胞溶酶体的选择性荧光标记物。
