Dali Prefectural Institute of Research and Control on Schistosomiasis, Dali 671000, Yunnan, China.
Clinical Medical College, Dali University, Dali 671000, Yunnan, China.
Parasitology. 2022 Sep;149(10):1349-1355. doi: 10.1017/S0031182022000609. Epub 2022 May 16.
The parasite is an important zoonotic parasite. The development of an animal model of 's life cycle is critical for studying the biological characteristics of the parasite in snails and mammals. Eggs of of bovine origin were cultured, and metacercariae were obtained after infection of snails. The life cycle system of was initiated in 2 different animals by orally infecting rabbits, SD rats and Kunming mice with the metacercariae. The animals' survival after infection, parasite migration in the animals and pathological damage to the liver were observed. We discovered that rabbits died due to acute suppurative hepatitis 6069 days after infection, and eggs were found in the feces on day 63 of infection. The liver of SD rats showed punctate lesions on day 3 of infection, and further changes occurred as the infection progressed. However, liver repair was observed at week 9. SD rats survived for more than a year after infection and continued the life cycle. The liver lesions in Kunming mice after infection were similar but more severe than those in SD rats. Death was observed on the 31st postinfection day. We discovered that while rabbits, SD rats and Kunming mice can all be used as animal models of , SD rats are more suitable experimental animals in terms of tolerance and pathological response.
寄生虫是一种重要的人畜共患寄生虫。研究寄生虫在蜗牛和哺乳动物中的生物学特性，发展其生命周期的动物模型至关重要。牛源的虫卵经培养后，感染蜗牛获得囊蚴。通过用囊蚴经口感染兔、SD 大鼠和昆明小鼠，在 2 种不同动物中启动了的生命周期系统。观察动物感染后的存活情况、寄生虫在动物体内的迁移以及对肝脏的病理损伤。我们发现，感染后 60-69 天，兔因急性化脓性肝炎死亡，感染后第 63 天粪便中发现虫卵。SD 大鼠感染后第 3 天肝脏出现点状病变，随着感染的进展，病变进一步发生，但在第 9 周观察到肝脏修复。SD 大鼠感染后存活超过 1 年，并继续生命周期。昆明小鼠感染后的肝脏病变与 SD 大鼠相似，但更严重。感染后第 31 天观察到死亡。我们发现，虽然兔、SD 大鼠和昆明小鼠均可作为的动物模型，但就耐受性和病理反应而言，SD 大鼠是更适合的实验动物。
