Keesey Ian W, Zhang Jin, Depetris-Chauvin Ana, Obiero George F, Gupta Abhishek, Gupta Nitin, Vogel Heiko, Knaden Markus, Hansson Bill S
Max Planck Institute for Chemical Ecology, Department of Evolutionary Neuroethology, Hans-Knöll-Straße 8, D-07745 Jena, Germany.
Department of Biochemistry and Biotechnology, Technical University of Kenya, Haille-Sellasie Avenue, Workshop Rd, 0200 Nairobi, Kenya.
iScience. 2022 Apr 6;25(5):104212. doi: 10.1016/j.isci.2022.104212. eCollection 2022 May 20.
Comparative analyses of multiple genomes are used extensively to examine the gains and losses of chemosensory receptors across the genus . However, few studies have delved into functional olfactory characteristics. Here we assess olfactory function across 20 species, and identify and describe several similar elements of evolution. We document (a) minor changes in functional ligands based on amino acid substitutions, (b) major changes in olfactory function or perhaps entire receptor replacements, and (c) that only a few receptors are subject to repeated changes, whereas 32 out of 37 OSNs are largely functionally conserved. In addition, we generate a robust model for identifying olfactory function using genomic data and comprehensive ligand-receptor combinations, which includes the prediction of binding pockets. Moreover, this study highlights that functional olfactory evolution does not affect all chemosensory receptors equally, and that ecological, evolutionary, and developmental forces repeatedly affect only a small subset of available receptor proteins.
多个基因组的比较分析被广泛用于研究整个属化学感应受体的得失情况。然而，很少有研究深入探讨功能性嗅觉特征。在此，我们评估了20个物种的嗅觉功能，并识别和描述了几个相似的进化元素。我们记录了：(a)基于氨基酸替换的功能性配体的微小变化；(b)嗅觉功能的重大变化或可能是整个受体的替换；以及(c)只有少数受体经历反复变化，而37个嗅觉感受神经元中有32个在很大程度上功能保守。此外，我们利用基因组数据和全面的配体-受体组合生成了一个用于识别嗅觉功能的可靠模型，其中包括结合口袋的预测。而且，这项研究强调功能性嗅觉进化对所有化学感应受体的影响并不相同，生态、进化和发育力量只会反复影响一小部分可用的受体蛋白。
