Jiao Caifeng
School of Horticulture, Anhui Agricultural University, Hefei, China.
Front Plant Sci. 2022 Apr 29;13:874338. doi: 10.3389/fpls.2022.874338. eCollection 2022.
The involvement of PpCBF6 in phytosulfokine α (PSKα)-ameliorated chilling injury (CI) by suppressing the expression of lipoxygenase 5 (LOX5) in peach fruit was revealed. The peaches were immersed in distilled water and PSKα solution. PSKα application inhibited the progression of CI index and weight loss, and the reduction of firmness and total soluble solids content in peaches. The endogenous PSKα accumulation and gene expression of PSK receptor 1 () and were up regulated by PSKα application. The superoxide anion (O ) production rate, hydrogen peroxide (HO) production and reactive oxygen species (ROS) content decreased by PSKα application. Furthermore, PSKα application reduced the gene expression of 12 and LOX activity. The gene expression of 6 was enhanced by PSKα application. Importantly, after PSKα application, among 12 , the decrease in gene expression of was the lowest, and among 6 , the increase in gene expression of was the highest. Further results suggested that PpCBF6 bound to the C-repeat/dehydration responsive element (CRT/DRE) motif in promoter, and repressed its transcription. Thus, PpCBF6 was involved in the PSKα-retarded CI by inhibiting the expression of in peaches.
研究揭示了PpCBF6通过抑制桃果实中脂氧合酶5（LOX5）的表达参与植物硫肽激素α（PSKα）改善的冷害（CI）过程。将桃子分别浸泡在蒸馏水和PSKα溶液中。施用PSKα可抑制CI指数的上升和重量损失，以及桃子硬度和总可溶性固形物含量的降低。施用PSKα可上调内源性PSKα的积累以及PSK受体1（）和的基因表达。施用PSKα可降低超氧阴离子（O）产生速率、过氧化氢（HO）产量和活性氧（ROS）含量。此外，施用PSKα可降低12的基因表达和LOX活性。施用PSKα可增强6的基因表达。重要的是，施用PSKα后，在12中，的基因表达下降幅度最小，在6中，的基因表达增加幅度最大。进一步的结果表明，PpCBF6与启动子中的C-重复/脱水响应元件（CRT/DRE）基序结合，并抑制其转录。因此，PpCBF6通过抑制桃子中的表达参与PSKα延缓的冷害过程。
