Non-vesicular Reference Laboratory, The Pirbright Institute, Pirbright, Woking, UK.
Methods Mol Biol. 2022;2503:119-132. doi: 10.1007/978-1-0716-2333-6_8.
Molecular methods are routinely used for the differential diagnosis and genetic characterization of viral diseases of livestock. Real-time PCR (qPCR) is known as the gold standard diagnostic method for most diseases and is also used for the detection of African swine fever virus (ASFV) DNA in clinical specimens. To determine the ASFV genotype or identify additional genome markers, endpoint PCR is usually performed on ASFV-positive specimens, followed by Sanger sequencing and data analysis. Here, we describe the ASFV genotyping method used at the OIE Reference Laboratory for ASF at the Pirbright Institute, United Kingdom.
分子方法常用于家畜病毒性疾病的鉴别诊断和遗传特征分析。实时 PCR（qPCR）被认为是大多数疾病的金标准诊断方法，也用于检测临床标本中的非洲猪瘟病毒（ASFV）DNA。为了确定 ASFV 基因型或识别其他基因组标记，通常在 ASFV 阳性标本上进行终点 PCR，然后进行 Sanger 测序和数据分析。在此，我们描述了英国 Pirbright 研究所的 OIE 非洲猪瘟参考实验室使用的 ASFV 基因分型方法。
