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评估用于透明软骨再生的组织工程产品有效性的现代实验方法。

Modern experimental methods for assessing the effectiveness of tissue-engineered products for hyaline cartilage regeneration.

作者信息

Bozhokin M S, Korneva Yu S, Bozhkova S A, Mikhaylova E R, Marchenko D M, Rakhimov B R, Nashchekina Y A, Khotin M G

机构信息

Center of Cell Technologies, Institute of Cytology Russian Academy of Science, Saint-Petersburg, Russia.

Centre of Traumatology and Orthopedics, Department of Wound Infection, Vreden National Medical Research, Saint-Petersburg, Russia.

出版信息

Front Bioeng Biotechnol. 2025 Jul 21;13:1595116. doi: 10.3389/fbioe.2025.1595116. eCollection 2025.

DOI:10.3389/fbioe.2025.1595116
PMID:40761544
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12318997/
Abstract

Hyaline cartilage (HC) is a specialized connective tissue that covers the surfaces of major joints and is characterized by its limited regenerative capacity. Modern therapeutic approaches to HC restoration often do not provide complete regeneration of damaged tissue. Developed tissue engineering methods show promise as effective approaches for restoring various types of HC damage. Due to the rapid evolution of various technologies in research practice, the range of methods available for analysis of TE constructs has expanded, including for the study of tissue engineering of hyaline cartilage (TEHC). Because of the complexity of the HC's structure, a whole range of methods is needed to assess characteristics of the scaffold, such as structure and strength. It is also important to study the behavior of cells inside the TE construct at all stages of cultivation, including post transplantation into the damaged area. The opacity of the scaffold and the complexity of its architecture often cause issues with the cell visualization and assessment of their viability. Therefore, there is a need to optimize each specific method for each specific scaffold. Despite the active study of TEHC, the results remain unsatisfactory. In this study, we have systematized data on the effectiveness and feasibility of methods to analyze structure, mechanical characteristics, cell interaction with the scaffold, and their ability to form new tissue before and after transplantation.

摘要

透明软骨（HC）是一种特殊的结缔组织，覆盖着主要关节的表面，其特点是再生能力有限。现代恢复透明软骨的治疗方法往往无法使受损组织完全再生。已开发的组织工程方法有望成为恢复各种类型透明软骨损伤的有效途径。由于研究实践中各种技术的快速发展，可用于分析组织工程构建体的方法范围有所扩大，包括用于透明软骨组织工程（TEHC）的研究。由于透明软骨结构的复杂性，需要一系列方法来评估支架的特性，如结构和强度。研究组织工程构建体内细胞在培养的各个阶段，包括移植到受损区域后的行为也很重要。支架的不透明度及其结构的复杂性常常导致细胞可视化和活力评估方面的问题。因此，需要针对每种特定的支架优化每种特定方法。尽管对透明软骨组织工程进行了积极研究，但结果仍不尽人意。在本研究中，我们整理了有关分析结构、力学特性、细胞与支架相互作用以及它们在移植前后形成新组织能力的方法的有效性和可行性的数据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfff/12318997/0398d5810521/fbioe-13-1595116-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfff/12318997/b4c6ff4bf989/fbioe-13-1595116-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfff/12318997/92025cde5f9b/fbioe-13-1595116-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfff/12318997/8b0ae9567cc6/fbioe-13-1595116-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfff/12318997/0398d5810521/fbioe-13-1595116-g010.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfff/12318997/806ca3247116/fbioe-13-1595116-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfff/12318997/7c57b30763cd/fbioe-13-1595116-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfff/12318997/73c468730e66/fbioe-13-1595116-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfff/12318997/20a40f676455/fbioe-13-1595116-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfff/12318997/89f8c90fa30b/fbioe-13-1595116-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfff/12318997/dba887cd46ac/fbioe-13-1595116-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfff/12318997/92025cde5f9b/fbioe-13-1595116-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfff/12318997/8b0ae9567cc6/fbioe-13-1595116-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cfff/12318997/0398d5810521/fbioe-13-1595116-g010.jpg

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