African Swine Fever Vaccinology Group, The Pirbright Institute, Pirbright, Woking, UK.
Division of Structural Biology, University of Oxford, Wellcome Trust Centre for Human Genetics, Headington, Oxford, UK.
Methods Mol Biol. 2022;2503:179-186. doi: 10.1007/978-1-0716-2333-6_13.
African swine fever virus is a cytolytic virus that leads to the apoptosis of both cultured cells and primary macrophages. Cell culture supernatants of virus-infected cells are routinely used for virological and immunological studies, despite differences in the biological behavior between such preparations and highly purified virus. In addition, more recent data suggests that exosomes containing viral proteins may be secreted from infected cells. While African swine fever virus can be purified through a number of methods, in our hands Percoll provides the most robust method of separating virus from cellular contaminants.
非洲猪瘟病毒是一种细胞溶解性病毒，可导致培养细胞和原代巨噬细胞凋亡。尽管病毒感染细胞的细胞培养上清液与高度纯化的病毒在生物学行为上存在差异，但它仍被常规用于病毒学和免疫学研究。此外，最近的数据表明，含有病毒蛋白的外泌体可能从感染细胞中分泌出来。虽然非洲猪瘟病毒可以通过多种方法进行纯化，但在我们的实验中，Percoll 提供了一种最有效的从细胞污染物中分离病毒的方法。
