非洲猪瘟病毒的纯化及物理化学特性

Purification and physicochemical characteristics of African swine fever virus.

作者信息

Black D N, Brown F

出版信息

J Gen Virol. 1976 Sep;32(3):509-18. doi: 10.1099/0022-1317-32-3-509.

Abstract

Methods for the purification of African swine fever virus (ASFV) and its dissection into two fractions are described. The difficulties which have been encountered previously in attempts to purify the virus, namely contamination with large amounts of cellular constituents and aggregation of the virus particles, have been overcome by treatment with Tween 80 and by the use of 1 M-NaCl in the sucrose gradients. Five major polypeptides, mol. wt. 10(3) X 125 (VP1), 76 (VP2), 50 (VP3), 44 (VP4) and 39 (VP5) were found in the purified particles. The virus was dissected by treatment with Nonidet NP 40 into (a) a fraction which had the appearance of an empty capsid shell and capsid shell and contained the polypeptides VP2 and VP3 and (b) a structure containing VP1 and VP4. The location of VP5 was not ascertained.

摘要

本文描述了非洲猪瘟病毒（ASFV）的纯化方法及其分为两个组分的过程。以往在纯化该病毒时遇到的困难，即大量细胞成分的污染和病毒颗粒的聚集，已通过吐温80处理以及在蔗糖梯度中使用1M氯化钠得以克服。在纯化颗粒中发现了五种主要多肽，分子量分别为10(3)×125（VP1）、76（VP2）、50（VP3）、44（VP4）和39（VP5）。通过用去氧胆酸钠NP 40处理，病毒被分解为：（a）一个看起来像空衣壳壳的组分，包含多肽VP2和VP3；（b）一个包含VP1和VP4的结构。VP5的位置尚未确定。