Ankara University, Faculty of Veterinary Medicine, Department of Microbiology, İrfan Baştuğ street, Dışkapı, Ankara, Turkey.
Ankara University, Faculty of Veterinary Medicine, Department of Genetics, İrfan Baştuğ street, Dışkapı, Ankara, Turkey.
Pol J Vet Sci. 2022 Mar;25(1):45-50. doi: 10.24425/pjvs.2022.140839.
Infectious bronchitis (IB) is an important disease that causes severe economic loses in the poultry industry worldwide. Furthermore, the spread of new variants poses a challenge for diagnosis and control of the disease. This study investigated the situation of infectious bronchitis virus (IBV), specifically the Israel variant-2 (IS var-2) also known as GI-23 genotype, in Turkey. Between 2014 and 2019, 214 flocks vaccinated against H120 from Marmara, Western Black Sea, and Inner Anatolia were examined, with 127 (59.3%) flocks testing positive for IBV, of which 92 (72.4%) were positive for IS var-2. Of the latter samples, 60 were randomly selected and subjected to full S1 gene sequencing. The analysis indicated that the field strain in Turkey was located on the same branch as the GI-23 genotype, which is one of the most frequently observed wild-type cluster found in the Middle East. The DNA similarities between the GI-23 isolates from 2014 to 2019 were 99%. In conclusion, the IS var-2 genotype has been circulating in broiler flocks in Turkey. It is recommended that establishing the vaccine strategy it should be considered the current circulating strains for the prevention and control of the disease among poultry.
传染性支气管炎（IB）是一种重要的疾病，它会给全球家禽业造成严重的经济损失。此外，新型变体的传播给疾病的诊断和控制带来了挑战。本研究调查了土耳其传染性支气管炎病毒（IBV）的情况，特别是以色列变体-2（IS var-2），也称为 GI-23 基因型。在 2014 年至 2019 年间，对来自马尔马拉、西黑海水域和安纳托利亚内陆的 214 个接种 H120 的鸡群进行了检查，其中 127 个（59.3%）鸡群的 IBV 检测呈阳性，其中 92 个（72.4%）为 IS var-2 阳性。在后者的样本中，随机选择了 60 个进行了全 S1 基因测序。分析表明，土耳其的田间菌株与 GI-23 基因型位于同一分支上，这是中东最常观察到的野生型簇之一。2014 年至 2019 年间 GI-23 分离株之间的 DNA 相似度为 99%。综上所述，IS var-2 基因型一直在土耳其的肉鸡群中传播。建议制定疫苗策略时，应考虑当前流行株，以预防和控制家禽中的疾病。
