Fujian Key Laboratory of Drug Target Discovery and Structural and Functional Research, School of Pharmacy, Fujian Medical University, Fuzhou, 350004, Fujian, China.
School of Clinical Medicine, Fujian Medical University, Fuzhou, 350004, Fujian, China.
Anal Bioanal Chem. 2022 Jul;414(17):4877-4884. doi: 10.1007/s00216-022-04110-7. Epub 2022 May 16.
As a kind of sensing and imaging fluorescent probe with the merit of low toxicity, good stability, and environment-friendly, silicon nanoparticles (SiNPs) are currently attracting extensive research. In this work, we obtained mitoxantrone-SiNPs (MXT-SiNPs) with green emission by one-pot synthesis under mild temperature condition. The antenna based on pyridoxal phosphate (PLP) was designed for light-harvesting to enhance the luminescence of MXT-SiNPs and to establish a novel sensing strategy for alkaline phosphatase (ALP). PLP transfers the absorbed photon energy to MXT-SiNPs by forming Schiff base. When PLP is dephosphorized by ALP, the released free hydroxyl group reacts with aldehyde group to form internal hemiacetal, which leads to the failure of Schiff base formation. Based on the relationship between antenna formation ability and PLP hydrolysis degree, the activity of ALP can be measured. A good linear relationship was obtained from 0.2 to 3.0 U/L, with a limit of detection of 0.06 U/L. Furthermore, the sensing platform was successfully used to detect ALP in human serum with recovery of 97.6-106.2%. The rational design of antenna elements for fluorescent nanomaterials can not only provide a new pathway to manipulate the luminescence, but also provide a new direction for fluorescence sensing strategy.
作为一种具有低毒性、良好稳定性和环保性的传感和成像荧光探针，硅纳米颗粒（SiNPs）目前引起了广泛的研究。在这项工作中，我们通过在温和的温度条件下一锅合成得到了具有绿色发射的米托蒽醌-SiNPs（MXT-SiNPs）。设计了基于吡哆醛磷酸盐（PLP）的天线用于光捕获，以增强 MXT-SiNPs 的发光，并建立了一种用于碱性磷酸酶（ALP）的新型传感策略。PLP 通过形成席夫碱将吸收的光子能量转移到 MXT-SiNPs 上。当 ALP 将 PLP 去磷酸化时，释放的游离羟基与醛基反应形成内半缩醛，从而导致席夫碱形成失败。基于天线形成能力与 PLP 水解程度的关系，可以测量 ALP 的活性。从 0.2 到 3.0 U/L 得到了良好的线性关系，检测限为 0.06 U/L。此外，该传感平台成功地用于人血清中 ALP 的检测，回收率为 97.6-106.2%。对于荧光纳米材料的天线元件的合理设计不仅可以提供一种新的途径来操纵发光，而且为荧光传感策略提供了一个新的方向。
