Pan Congjie, Lu Meicheng, Ma Longfei, Wu Mingxia
School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, 450046, China.
Henan Engineering Research Center of Modern Chinese Medicine Research, Development and Application, Zhengzhou, 450046, China.
J Fluoresc. 2024 Aug 26. doi: 10.1007/s10895-024-03906-3.
In this paper, blue fluorescent silicon nanoparticles (SiNPs) with outstanding optical properties and robust stability were synthesized by a simple one-step hydrothermal method. By introducing red emissive rhodamine B (RhB) into SiNPs solution, a dual emission nanoprobe (SiNPs@RhB) was constructed, which showed excellent pH stability, salt resistance and photobleaching resistance. The SiNPs@RhB probe could emit two peaks at 444 nm and 583 nm under 365 nm excitation. It was found that the fluorescence intensity of the two emission peaks decreased in different degrees with the addition of different concentrations of kaempferol (Kae). According to this phenomenon, a novel ratiometric fluorescence method was established for the detection of Kae via utilizing SiNPs@RhB as nanoprobe. The detection range and limit of detection (LOD) were 0.5 ~ 150 µM and 0.24 µM, respectively. The ratiometric fluorescence method exhibited the superiority of rapid detection, excellent stability, wide linear range and high sensitivity. The detection mechanism was studied by ultraviolet visible absorption spectra, fluorescence spectra and fluorescence lifetime. Furthermore, the method was applied to the detection of Kae in real samples (kaempferia powder, sea buckthorn granules and sea buckthorn dry emulsion).
本文通过简单的一步水热法合成了具有优异光学性能和强稳定性的蓝色荧光硅纳米颗粒（SiNPs）。通过将红色发射罗丹明B（RhB）引入SiNPs溶液中，构建了一种双发射纳米探针（SiNPs@RhB），其表现出优异的pH稳定性、耐盐性和抗光漂白性。SiNPs@RhB探针在365nm激发下可在444nm和583nm处发射两个峰。研究发现，随着不同浓度山奈酚（Kae）的加入，两个发射峰的荧光强度均有不同程度的降低。基于此现象，建立了一种以SiNPs@RhB为纳米探针检测Kae的新型比率荧光法。该方法的检测范围和检测限（LOD）分别为0.5~150μM和0.24μM。该比率荧光法具有检测快速、稳定性好、线性范围宽和灵敏度高的优势。通过紫外可见吸收光谱、荧光光谱和荧光寿命对检测机理进行了研究。此外，该方法还应用于实际样品（山奈粉、沙棘颗粒和沙棘干乳剂）中Kae的检测。
