College of Food Science and Engineering, Ocean University of China, Qingdao 266003, PR China.
College of Food Science and Engineering, Ocean University of China, Qingdao 266003, PR China.
Food Chem. 2022 Oct 1;390:133194. doi: 10.1016/j.foodchem.2022.133194. Epub 2022 May 12.
Histamine is the prime culprit of toxicity resulting from seafood consumption, whereas conventional detection methods are not convenient to meet the needs of rapid histamine analysis nowadays. Based on upconversion nanoparticles (UCNPs) and inner filter effects (IFE), a novel ratiometric fluorescence nanosystem was developed for the efficient detection of histamine. Through pre-treatment with solid-phase extraction (SPE) and colorific azo coupling reaction of histamine, the fluorescence of UCNPs at 548 nm was quenched, while fluorescence at 664 nm was unchanged. Thus, ratiometric fluorescence I/I was inversely proportional to histamine concentration at a wide range of 10-200 mg/L. The detection limit was 7.34 mg/L, one order of magnitude lower than that of the traditional colorimetric method (25 mg/L). In addition, such a convenient and environment-friendly detection system was further employed to quantify the histamine in fish, shrimp, and shellfish samples, showing excellent application potential in seafood safety.
组氨酸是导致海鲜食用中毒的主要元凶，而传统的检测方法并不方便，无法满足现今快速分析组氨酸的需求。基于上转换纳米粒子（UCNPs）和内滤效应（IFE），开发了一种新型的比率荧光纳米系统，用于高效检测组氨酸。通过固相萃取（SPE）预处理和组氨酸的显色偶联反应，UCNPs 在 548nm 处的荧光被猝灭，而 664nm 处的荧光保持不变。因此，在较宽的 10-200mg/L 范围内，比率荧光 I/I 与组氨酸浓度呈反比关系。检测限为 7.34mg/L，比传统比色法（25mg/L）低一个数量级。此外，这种方便且环保的检测系统还进一步用于定量测定鱼类、虾类和贝类样品中的组氨酸，在海鲜安全领域具有极好的应用潜力。
