Special Infectious Agents Unit BSL-3, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, 21589, Saudi Arabia; Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, 21589, Saudi Arabia.
Special Infectious Agents Unit BSL-3, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, 21589, Saudi Arabia; Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, 21589, Saudi Arabia; Department of Nursing, Faculty of Al-Qunfudah Health Sciences, Umm Al-Qura University, Makkah, 28821, Saudi Arabia.
Int J Infect Dis. 2022 Aug;121:130-137. doi: 10.1016/j.ijid.2022.05.027. Epub 2022 May 13.
Acute respiratory tract infections (ARIs) due to human rhinoviruses (HRVs) are common in pilgrims during the annual Hajj pilgrimage. The objective of this study was to investigate the genetic diversity of HRV among pilgrims with respiratory symptoms during Hajj 2019.
HRV infection was detected using multiplex real-time reverse transcription polymerase chain reaction. Cycle sequencing was performed on positive samples and the sequences were subjected to phylogenetic analysis.
A total of 19 HRV-positive respiratory samples were sequenced. All three serotypes of HRV were identified: HRV-A (13; 68.42%) was more common than HRV-B (2; 10.53%) and HRV-C (4; 21.05%). HRV-A species were found to be of genotypes A101, A21, A30, A57, A23, A60, and A11. HRV-B species belonged to genotypes B4 and B84, and HRV-C species were of genotypes C15, C3, and C56.
Sequencing studies of respiratory tract viruses in pilgrims are important. We provide preliminary evidence of high diversity of HRV genotypes circulating in pilgrims in a restricted area during Hajj. This requires further clinical and sequencing studies of viral pathogens in larger cohorts of overseas and local pilgrims.
在每年的朝觐期间，人类鼻病毒（HRV）引起的急性呼吸道感染（ARI）在朝圣者中很常见。本研究旨在调查 2019 年朝觐期间有呼吸道症状的朝圣者中 HRV 的遗传多样性。
使用多重实时逆转录聚合酶链反应检测 HRV 感染。对阳性样本进行循环测序，并对序列进行系统发育分析。
共测序了 19 份 HRV 阳性呼吸道样本。鉴定出了三种 HRV 血清型：HRV-A（13；68.42%）比 HRV-B（2；10.53%）和 HRV-C（4；21.05%）更常见。发现 HRV-A 种属于基因型 A101、A21、A30、A57、A23、A60 和 A11。HRV-B 种属于基因型 B4 和 B84，而 HRV-C 种属于基因型 C15、C3 和 C56。
对朝圣者呼吸道病毒进行测序研究很重要。我们提供了在朝觐期间，在一个有限的区域内，HRV 基因型在朝圣者中广泛传播的初步证据。这需要对海外和当地更大的朝圣者队列中的病毒病原体进行进一步的临床和测序研究。
