State Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An, Shandong, China; and Collaborative Innovation Center of Fruit and Vegetable Quality and Efficient Production in Shandong, Tai-An, China.
Funct Plant Biol. 2022 Aug;49(9):799-809. doi: 10.1071/FP21146.
Anthocyanins play important roles in plant secondary metabolism. Although previous studies have identified many transcription factors (TFs) that participate in the synthetic pathway of anthocyanins, the regulation mechanism of the pathway remain poorly understood. In this study, we identified a WRKY Group IIc TF, MdWRKY75, which contained a typical WRKYGQK heptapeptide sequence and a C2 H2 -zinc finger structure. Subcellular localisation assays found that MdWRKY75 was located in the nucleus. Overexpression of MdWRKY75 promoted the accumulation of anthocyanins in apple (Malus domestica L.) 'Orin' calli. MdWRKY75 mainly stimulated the accumulation of anthocyanins by binding to the promoter of MYB transcription factor, MdMYB1 . Our research could provide new insights into how WRKY TFs regulate the accumulation of anthocyanins in apples.
花青素有参与植物次生代谢的重要作用。虽然之前的研究已经确定了许多参与花色苷合成途径的转录因子（TFs），但该途径的调控机制仍知之甚少。在这项研究中，我们鉴定了一个 WRKY 组 IIc TF，MdWRKY75，它包含一个典型的 WRKYGQK 七肽序列和一个 C2 H2-锌指结构。亚细胞定位实验发现 MdWRKY75 位于细胞核内。过表达 MdWRKY75 促进了苹果（Malus domestica L.）‘Orin’愈伤组织中花色苷的积累。MdWRKY75 主要通过与 MYB 转录因子 MdMYB1 的启动子结合来刺激花色苷的积累。我们的研究为 WRKY TFs 如何调节苹果中花色苷的积累提供了新的见解。
