Zuo Zhidan, Ma Huihui, Li Longteng, Qian Jialin, Zhang Minghui, Li Xiang, Sheng Yeshun, Wang Yuxin
College of Life Sciences, Zaozhuang University, Zaozhuang 277160, China.
Key Laboratory of Sweet Potato Biology and Biotechnology of Ministry of Agriculture and Rural Affairs, College of Agronomy & Biotechnology, China Agricultural University, Beijing 100193, China.
Genes (Basel). 2025 Feb 24;16(3):266. doi: 10.3390/genes16030266.
BACKGROUND/OBJECTIVES: Stay-green proteins (SGRs) play a vital role in regulating plant chlorophyll degradation and senescence. However, this gene family has not been explored in species and sweet potato.
A total of 19 family genes () were identified using Basic Local Alignment Search Tool (BLAST) methods. The proteins' physiological properties, evolutionary and phylogenetic relationships, conserved domain and motifs, gene structures, collinearity, and promoter -elements were systematically analyzed. Moreover, expression patterns and protein interaction network analyses were performed for sweet potato.
In this study, we identified 19 in six species. These were divided into four subgroups according to their phylogenetic relationships. Domian analysis revealed that SGRs had the conserved "stay-green" domain. Gene structure analysis showed that had similar structures. The collinearity analysis revealed that the originated from two genes, with one gene undergoing duplication during evolution history; moreover, the experienced rearrangement throughout the evolutionary process in the species. -elements related to pigment biosynthesis and hormone and stress responses were found. In addition, expression pattern analysis showed that , especially , , and , might play an important role in pigment accumulation. The could also respond to stress responses (i.e., cold, drought, and salt) and take part in hormone crosstalk (i.e., abscisic acid (ABA), methyl jasmonate (MeJA), salicylic acid (SA)).
Taken together, the findings of this study provide new insights for further understanding the functions of and candidate genes for pigment accumulation and stress tolerance in sweet potatoes.
背景/目的：保持绿色蛋白（SGRs）在调节植物叶绿素降解和衰老过程中起着至关重要的作用。然而，该基因家族在[物种名称]和甘薯中尚未得到研究。
使用基本局部比对搜索工具（BLAST）方法共鉴定出19个[基因家族名称]家族基因。对这些蛋白质的生理特性、进化和系统发育关系、保守结构域和基序、基因结构、共线性以及启动子元件进行了系统分析。此外，还对甘薯进行了表达模式和蛋白质相互作用网络分析。
在本研究中，我们在6个[物种名称]物种中鉴定出19个[基因家族名称]。根据系统发育关系，这些[基因家族名称]被分为四个亚组。结构域分析表明，SGRs具有保守的“保持绿色”结构域。基因结构分析表明，[基因家族名称]具有相似的结构。共线性分析表明，[基因家族名称]起源于两个基因，其中一个基因在进化历史中发生了复制；此外，[基因家族名称]在[物种名称]物种的整个进化过程中经历了重排。发现了与色素生物合成以及激素和应激反应相关的元件。此外，表达模式分析表明，[基因家族名称]，尤其是[具体基因名称1]、[具体基因名称2]和[具体基因名称3]，可能在色素积累中起重要作用。[基因家族名称]还可以响应应激反应（即寒冷、干旱和盐胁迫）并参与激素互作（即脱落酸（ABA）、茉莉酸甲酯（MeJA）、水杨酸（SA））。
综上所述，本研究结果为进一步了解[基因家族名称]的功能以及甘薯中色素积累和胁迫耐受性的候选基因提供了新的见解。
