Felipez Winder, Villavicencio Jennifer, Nizolli Valeria Oliveira, Pegoraro Camila, da Maia Luciano, Costa de Oliveira Antonio
Instituto de Agroecología y Seguridad Alimentaria, Facultad de Ciências Agrárias, Universidad San Francisco Xavier de Chuquisaca-USFX, Casilla, Correo Central, Sucre 1046, Bolivia.
Plant Genomics and Breeding Center, Departamento de Fitotecnia, Faculdade de Agronomia Eliseu Maciel, Universidade Federal de Pelotas-UFPel, Pelotas CEP 96010-900, RS, Brazil.
Plants (Basel). 2023 Sep 5;12(18):3176. doi: 10.3390/plants12183176.
WRKY transcription factor genes compose an important family of transcriptional regulators that are present in several plant species. According to previous studies, these genes can also perform important roles in bilberry ( L.) metabolism, making it essential to deepen our understanding of fruit ripening regulation and anthocyanin biosynthesis. In this context, the detailed characterization of these proteins will provide a comprehensive view of the functional features of genes in different plant organs and in response to different intensities of light. In this study, the investigation of the complete genome of the bilberry identified 76 genes that were evaluated and distributed in all twelve chromosomes. The proteins encoded by these genes were classified into four groups (I, II, III, and IV) based on their conserved domains and zinc finger domain types. Fifteen pairs of genes in segmental duplication and four pairs in tandem duplication were detected. A cis element analysis showed that all promoters of the genes contain at least one potential cis stress-response element. Differential expression analysis of RNA-seq data revealed that genes from bilberry show preferential or specific expression in samples. These findings provide an overview of the functional characterization of these proteins in bilberry.
WRKY转录因子基因构成了一个重要的转录调节因子家族，存在于多种植物物种中。根据以往的研究，这些基因在越橘（Vaccinium myrtillus L.）的新陈代谢中也发挥着重要作用，因此加深我们对果实成熟调控和花青素生物合成的理解至关重要。在此背景下，对这些蛋白质的详细表征将全面了解基因在不同植物器官以及对不同光照强度响应中的功能特性。在本研究中，通过对越橘全基因组的研究，鉴定出76个WRKY基因，这些基因分布在所有12条染色体上并进行了评估。根据其保守结构域和锌指结构域类型，这些基因编码的蛋白质被分为四组（I、II、III和IV）。检测到15对基因存在片段重复，4对基因存在串联重复。顺式元件分析表明，所有WRKY基因的启动子至少包含一个潜在的顺式胁迫响应元件。对RNA测序数据的差异表达分析表明，越橘中的WRKY基因在样本中表现出优先或特异性表达。这些发现概述了越橘中这些蛋白质的功能特征。
