Centre of Polymer Systems, Tomas Bata University in Zlin, Zlin, Czech Republic.
Faculty of Technology, Tomas Bata University in Zlin, 760 01, Zlin, Czech Republic.
Sci Rep. 2022 May 16;12(1):8065. doi: 10.1038/s41598-022-11678-8.
Bio-inspired conductive scaffolds composed of sodium hyaluronate containing a colloidal dispersion of water-miscible polyaniline or polypyrrole particles (concentrations of 0.108, 0.054 and 0.036% w/w) were manufactured. For this purpose, either crosslinking with N-(3-dimethylaminopropyl-N-ethylcarbodiimide hydrochloride and N-hydroxysuccinimid or a freeze-thawing process in the presence of poly(vinylalcohol) was used. The scaffolds comprised interconnected pores with prevailing porosity values of ~ 30% and pore sizes enabling the accommodation of cells. A swelling capacity of 92-97% without any sign of disintegration was typical for all samples. The elasticity modulus depended on the composition of the scaffolds, with the highest value of ~ 50 kPa obtained for the sample containing the highest content of polypyrrole particles. The scaffolds did not possess cytotoxicity and allowed cell adhesion and growth on the surface. Using the in vivo-mimicking conditions in a bioreactor, cells were also able to grow into the structure of the scaffolds. The technique of scaffold preparation used here thus overcomes the limitations of conductive polymers (e.g. poor solubility in an aqueous environment, and limited miscibility with other hydrophilic polymer matrices) and moreover leads to the preparation of cytocompatible scaffolds with potentially cell-instructive properties, which may be of advantage in the healing of damaged electro-sensitive tissues.
采用了两种方法来制备含有水混溶性聚（苯胺）或聚（吡咯）胶体分散体（浓度分别为 0.108%、0.054%和 0.036%w/w）的透明质酸钠基仿生导电支架：一种是使用 N-（3-二甲氨基丙基）-N-乙基碳二亚胺盐酸盐和 N-羟基琥珀酰亚胺交联，另一种是在聚乙烯醇存在下进行冻融处理。支架由相互连通的孔组成，具有 30%左右的高孔隙率和适合细胞生长的孔径。所有样品的溶胀率都在 92%-97%之间，没有任何解体的迹象。弹性模量取决于支架的组成，含有最高含量聚吡咯颗粒的样品的弹性模量约为 50kPa。支架没有细胞毒性，可以促进细胞黏附和生长。在生物反应器中模拟体内条件，细胞也能够长入支架结构中。因此，这里使用的支架制备技术克服了导电聚合物的局限性（例如在水环境中溶解度差，与其他亲水性聚合物基质的相容性有限），并且可以制备出具有潜在细胞导向性的细胞相容性支架，这可能有利于受损电敏感组织的修复。
