Sramek S J, Wallow I H, Bindley C, Sterken G
Invest Ophthalmol Vis Sci. 1987 Mar;28(3):500-5.
Previous studies of fibronectin (FN) distribution in eye tissue have relied on immunofluorescence (IF) techniques on frozen sections, and have not included the rat. Using rat eyes, a technique was developed for immunoperoxidase (IP) staining of formalin fixed paraffin embedded material, and the results were compared to those obtained by IF. IP was technically more difficult, and required pepsinization of tissue after formalin fixation to obtain consistent results. The optimum pepsin time varied for different structures in the eye. IP offers better tissue preservation and stain resolution. Results with IF were consistent with those observed with the newly applied IP. The distribution of FN in rat eyes was similar though not identical to that reported in other species. Prominent stain was observed in the conjunctiva, basement membrane of the corneal epithelium, corneal stroma, anterior aspect of Descemet's membrane, trabecular meshwork, perivascular stroma of the ciliary body, choroid and retinal blood vessels. Lens structures, vitreous, and internal limiting membrane of the retina were negative.
以往关于纤连蛋白（FN）在眼组织中分布的研究依赖于对冰冻切片进行免疫荧光（IF）技术，且未涉及大鼠。利用大鼠眼睛，开发了一种对福尔马林固定石蜡包埋材料进行免疫过氧化物酶（IP）染色的技术，并将结果与通过IF获得的结果进行比较。IP在技术上更具难度，且需要在福尔马林固定后对组织进行胃蛋白酶处理以获得一致的结果。眼睛中不同结构的最佳胃蛋白酶处理时间各不相同。IP能提供更好的组织保存和染色分辨率。IF的结果与新应用的IP所观察到的结果一致。大鼠眼睛中FN的分布与其他物种报道的相似但不完全相同。在结膜、角膜上皮基底膜、角膜基质、Descemet膜前部、小梁网、睫状体血管周围基质、脉络膜和视网膜血管中观察到明显染色。晶状体结构、玻璃体和视网膜内界膜呈阴性。
